蛋白酪氨酸磷酸酶基因启动子过甲基化与喉癌的关系  

The relationship between hypermethylation of the PTEN promoter and laryngeal squamous cell carcinoma

作　　者：白伟良[1] 李巍[1] 陈晓秋[1] 王铁[1] 

机构地区：[1]中国医科大学附属第二医院耳鼻咽喉科,沈阳110004

出　　处：《临床耳鼻咽喉科杂志》2006年第6期254-257,共4页Journal of Clinical Otorhinolaryngology

摘　　要：目的:探讨蛋白酪氨酸磷酸酶(PTEN)基因启动子过甲基化与喉癌的关系。方法:采用过甲基化特异性PCR和RT-PCR法分析喉癌(喉癌组)及正常喉组织(对照组)PTEN基因启动子过甲基化及其mRNA表达情况。结果:喉癌组有16例(40.0%)PTEN基因启动子过甲基化;对照组未发现过甲基化。对照组PTEN基因mRNA的高、中、低表达率及阴性率分别为77.8%(7/9)、22.2%(2/9)、0和0,淋巴结转移组分别为26.7%(4/15)、33.3%(5/15)、26.7%(4/15)和13.3%(2/15),两组比较,PTEN mRNA表达差异有统计学意义(P<0.05)。病理高分化PTEN基因mRNA高、中、低表达率及阴性率分别为60.0%(6/10)、30.0%(3/10)、10.0%(1/10)和0,病理低分化mRNA分别为16.7%(2/12)、25.0%(3/12)、41.7%(5/12)和16.7%(2/12),两者比较,其mRNA表达差异有统计学意义(P<0.05)。PTEN基因在喉癌组织中,mRNA呈现低表达的10例中有7例(70.0%)出现启动子过甲基化,mRNA呈现高表达的17例中仅有4例(23.5%)出现启动子过甲基化;PTEN基因mRNA高表达与低表达在启动子过甲基化率的差异有统计学意义(P<0.05)。结论:PTEN基因启动子过甲基化是该基因在喉癌中失活的原因之一,PTEN基因表达下降与喉癌淋巴结转移及病理低分化有关。Objective：To explore the relationship between hypermethylation of the PTEN gene promoter and laryngeal squamous cell carcinoma（LSCC）. Method： Promoter hypermethylation and mRNA expression were detected by methylation specific PCR and RT-PCR in LSCC and normal laryngeal tissue. Result：Among the 40 patients with LSCC, hypermethylation of PTEN gene promoter was detected in 16 cases （40.0%）. Among the nine normal laryngeal tissues, hypermethylation of PTEN gene promoter was detected in no case. The high and moderate expression rates of PTEN gene mRNA in nine normal laryngeal tissues were 77.8% （7/9） and 22.2% （2/9）, respectively; The high, moderate, low and negative expression rates of PTEN mRNA in 15 laryngeal cancers with lymph node metastasis were 26.7%（4/15）, 33.3%（5/15）, 26.7%（4/15） and 13.3%（2/15）, respectively. The PTEN mRNA expression in normal laryngeal tissues was higher than that in the laryngeal cancers with lymph node metastasis （ P〈0.05）. The high, moderate and low expression rates of PTEN mRNA in 10 highly differentiated laryngeal cancers were 60.0%（6/10）, 30.0%（3/10） and 10.0%（1/10）, respectively; The high, moderate, low and negative expression rates of PTEN mRNA in 12 low differentiated laryngeal cancers were 16. 7% （2/12）, 25.0%（3/12）, 41.7%（5/12） and 16.7%（2/12）, respectively. The differences of PTEN mRNA expression between high and low differentiation laryngeal cancers were statistically significant（ P〈0.05）. The hypermethylation rate of PTEN gene promoter in the low mRNA expression laryngeal cancers was 70.0 %（ 7/10）, the hypermethylation rate of PTEN gene promoter in the high mRNA expression laryngeal cancers was 23.5 %（4/17）. The hypermethylation rate of PTEN gene promoter in the low mRNA expression laryngeal cancers was higher than that in the high mRNA expression laryngeal cancers （ P〈0.05）. Conclusion.. Hypermethylation of PTEN gene promoter is associated with loss of its transcription in laryngeal sq

关 键 词：喉肿瘤 蛋白酪氨酸磷酸酶 蛋白酪氨酸磷酸酶基因启动子 过甲基化 

分 类 号：R739.65[医药卫生—肿瘤]