4-1BBL介导的逆向信号对人外周血T细胞体外活化的调节作用  

The reverse signal mediation of regulates activation of human peripheral blood T cell by 4-1BBL in vitro

作　　者：居颂文[1] 居颂光[1] 仇红霞[1] 王凤鸣[1] 王明元[1] 胡玉敏[1] 刘彤[1] 刘高勤[1] 张学光[1] 

机构地区：[1]苏州大学医学生物技术研究所,苏州大学附属第一人民医院临床免疫实验室,215007

出　　处：《中华微生物学和免疫学杂志》2006年第2期101-104,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然基金青年科学基金项目(30400394);江苏省教育厅博士生创新计划(50320025)

摘　　要：目的探讨表达于活化T细胞上的4-1BBL分子对T细胞的调节作用及可能机制。方法鼠抗人4-1BBL单克隆抗体1F1加入CD3单抗联合CD28单抗激发的T细胞培养体系。细胞计数分析T细胞的增殖;PI/AnnexinⅤ双染法分析T细胞的凋亡;ELISA法测定培养上清中细胞因子的分泌水平;流式细胞仪分析T细胞的表型。结果单抗1F1能够有效地抑制活化T细胞的体外增殖并诱导其凋亡,同时单抗1F1还可抑制活化T细胞分泌IL-2和IFN-γ及上调T细胞表面CD95和PD-1分子的表达。结论在T细胞活化过程中,活化T细胞表达的4-1BBL分子通过逆向信号抑制其细胞因子的分泌和上调负性调控分子,进而抑制T细胞的过度活化和下调T细胞介导的免疫应答。Objective To analyze the role of 4-1BBL, expressed in the regulation of human peripheral blood T cell activation. Methods Apoptotic T cells were evaluated by propidium iodide uptake and Annexin V binding. Cytokine concentrations in supernatants were determined by ELISA. Phenotypic analyses were performed with direct immunofluorescence techniques in a FACS. Reultss McAb 1F1 inhibited T cell proliferation and induced T cells apoptosis. In addition, McAb 1F1 suppressed the secretion of IL-2 and IFN-γ, and upregulated the expression of CD95 and PD-1.Conclusion 4-1BBL reverse signal prevent T cell activation and downregulate immune responses mediated by T cell via inhibiting secretion of cytokines and uprregulating negative costimulatory molecule.

关 键 词：4-1BBL 逆向信号 共刺激分子 T细胞活化 

分 类 号：R392[医药卫生—免疫学]