尼美舒利对胃癌细胞BGC823 COX-2、VEGF表达的影响  

Effection of Nimesulide on expression of COX-2 and VEGF in human gastric-carcinoma cell line BGC-823

作　　者：张菊[1,2] 

机构地区：[1]重庆医科大学附属一医院肿瘤科,重庆400016 [2]重庆医科大学附属一医院消化科,重庆400016

出　　处：《世界肿瘤杂志》2005年第4期262-264,273,F0003,共5页Tumour Journal of the World

摘　　要：目的 研究尼美舒利（Nimesulide）对人胃癌细胞株BGC-823增殖和凋亡的干预作用，揭示其抗癌机制。方法 采用MTT法、流式细胞仪等检测细胞增殖、细胞周期和凋亡率以及细胞的形态学改变，同时检测尼美舒利对胃癌细胞COX-2、VEGF表达的影响。结果 尼美舒利抑制胃癌细胞的IC50μM／L。增殖抑制率达到80．31％，流式细胞仪分析发现100-200μM／L的尼美舒利作用48h后，细胞被阻滞于S期，凋亡率分别为5．69％，10．58％；光学显微镜观察到凋亡典型的形态学特征；免疫细胞化学结果显示尼美舒利作用后能降低COX2、VEGF蛋白表达，与对照组比较，具有非常显著性差异（P〈0．01）。结论 尼美舒利能显著抑制人胃癌细胞株BGC-823的生长，此作用可能与降低COX2、VEGF蛋白表达有关。Objective To analyze the growth-inhibitory effects of nimesulide on the human gastriccarcinoma cell line BGC823, and speculate its mechanism. Methods Proliferation of the cells was detected by using of MTT assay; Morphological changes of cells were observed by microscopy; cell cycle and apopotosis were analysed by means of flow cytometry; Expressions of COX-2 and VEGF were investigated by immunocytochemistry. Results Nimesulide had a significant inhibiting effect to growth and proliferation of human gastric cancer line BGC823 in vitro in a does-dependent manner. The IC50μM/L is after exposure Nimesulide for 48h. Microscopy provided that apoptosis occurred in the gastric cancer cells in treatment groups. FCM showed that Nimesulide arrested BGC823 cells at the Gz/M phase and the apoptosis rate was increased. Immunocytochemistry showed the expressions of COX-2 and VEGF were significantly inhibited by 100um Nimesulide. Conclusions The results showed Nimesulide markedly inhibited the growth of BGC823, and its mechanism maybe inhibit to express COX-2 and VEGF contributed to the neoplasm cell apoptosis.

关 键 词：尼美舒利 凋亡 中效原理 COX2 VEGF 

分 类 号：R730.21[医药卫生—肿瘤]