荧光定量RT-PCR检测食管癌外周血CK19mRNA和CK20mRNA表达  

作　　者：赵强[1] 陈奇勋[1] 冯建国[2] 朱赤红[2] 顾琳惠[2] 姜志明[2] 

机构地区：[1]浙江省肿瘤医院胸腹外科,310022 [2]浙江省肿瘤研究所,310022

出　　处：《浙江临床医学》2006年第3期227-228,共2页Zhejiang Clinical Medical Journal

摘　　要：目的应用荧光定量RT-PCR(Fluorogenic quantitative reverse transcriptase polymerase chain reaction,RT-PCR)检测食管癌外周血中微转移情况。方法用荧光定量RT-PCR方法检测38例食管癌患者外周血CK19mRNA和CK20mRNA表达。结果38例食管癌患者外周血中CK19mRNA检测阳性14例,阳性率36.84%;CK20mRNA检测阳性6例,阳性率15.79%;两者任一阳性18例,阳性率47.37%。结果显示外周血CK19mRNA、CK20mRNA的表达与临床分期、分化程度和淋巴结转移相关,与性别、年龄、病理类别无关。结论用荧光定量RT-PCR法联合检测食管癌患者外周血CK19mRNA和CK20mRNA有助于筛选亚临床转移和指导临床治疗方案的制定。Objective To detect the micrometastases status in peripheral blood of esophageal cancer patients by fluorogenic quantitative reverse transcripition-polymensse. chain reaction（RT-PCR）. Methods CK19mRNA and CK20mRNA expression in peripheral blood were detected in 38 cass of esophageal cancer by fluomgenic quantitative RT - PCR. Results Positive CK19mRNA and CK20mRNA were detected in peripheral blood in 14 and 6 of 38 patients with esophageal Cancer. The positive rate was 36.84% and 15.79%, respectively. Statisical analysis indicated that expression of CK19mRNA and CK20mRNA in peripheral blood were not correlated with gender, age and pathological type of the cancer, but had significant differenec in clinical TNM stage , lymphnode metastasis status and cell differentiation. Conclusion RT - PCR amplification of CK19mRNA and CK2.0mRNA is a sensitive and special method to detect early haenmtogenous disseronination of cancer cells for patient with esophageal cancer, and may lead to early diagnosis of patients with subclinical metastasis in circulation.

关 键 词：RT-PCR 食管癌 CK19MRNA CK20MRNA 微转移 外周血 

分 类 号：R735.1[医药卫生—肿瘤]