hBD_2修饰真皮多能干细胞促进感染创面修复的初步实验研究  

Repair effect of hBD_2-modified dermal multipotent stem cells transplantation on infected wound

作　　者：李楠[1] 肖桃元[2] 粟永萍[1] 徐辉[1] 王军平[1] 宗兆文[1] 董世武[1] 冉新泽[1] 刘志君[1] 

机构地区：[1]第三军医大学军事预防医学院防原医学教研室 [2]第三军医大学高原军事医学系中心实验室,重庆400038

出　　处：《第三军医大学学报》2006年第5期425-427,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目("973"项目)(2005CB522605);国家自然科学基金资助项目(30300185)~~

摘　　要：目的观察人β防御素2(humanβdefensin2,hBD2)修饰的大鼠真皮多能干细胞(dermalmultipotentstemcells,dMSCs)移植后对细菌感染创面修复的影响。方法制作大鼠全层皮肤切割伤伴感染模型,点状注射的方法将hBD2腺病毒载体转染修饰的dMSCs移植到伤口,通过测定痂下菌量及创面愈合面积百分率和创面愈合时间等评价hBD2修饰的dMSCs局部移植的促愈效果。结果和局部单纯移植dMSCs组和单纯损伤组相比,hBD2修饰的dMSCs移植组痂下菌含量低(P<0.05),且创面愈合时间明显缩短(P<0.05)。结论hBD2修饰的dMSCs可有效地促进大鼠感染创面的修复。Objective To observe the repair effect of human β-defensin 2 （hBD2 ）-modified rat dermal muhipotent stem ceils （dMSCs） transplantation on infected wound. Methods Thirty Wistar rats were excised a piece of whole-layer back skin, 3 mm in diameter, then infected the wound with 1 × 10^8/ml pseudomonas aeruginosa 1 ml, then the rats were injected on the wounded back respectively with dMSCs modified by hBD2 （ n = 10）, or pure dMSCs （n = 10） or none as control （n = 10）. The repair effect was evaluated by observing the amount of bacteria under the scar, wound healing time and the percentage of remaining wound area. Results The amount of bacteria under the scar in rats that were transplanted with dMSCs modified by hBD2 was less than that in rats transplanted with dMSCs or controls （ P 〈 0. 05 ）, as well as the wound healing time （ P 〈 0. 05 ）. Conclusion Transplantation of dMSCs modified by hBD2 could effectively enhance the repair of infected wound in rats.

关 键 词：人Β防御素2 真皮多能干细胞 创面修复 感染 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R630.5[医药卫生—基础医学]