Transcription Factors Ets2 and Sp1 Act Synergistically with Histone Acetyltrans-ferase p300 in Activating Human Interleukin-12 p40 Promoter  

作　　者：Hai-Jing SUN Xin XU Xiu-Li WANG Liang WEI Fen LI Jun LU Bai-Qu HUANG 

机构地区：[1]Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China [2]Beijing Normal University, Beijing 100875, China

出　　处：《Acta Biochimica et Biophysica Sinica》2006年第3期194-200,共7页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China (30571698);the Major State Basic Research Project of China (2005CB522404)

摘　　要：There has been considerable interest in researching the regulatory mechanisms that control the synthesis of interleukin （IL）-12, which plays a central role in the differentiation of T-helper-1 cells. In this study, we performed a series of transient transfection experiments designed to elucidate the functional relationship between the IL-12 promoter-specific transcription factors （Ets2 and Spl） and histone acetylation modification in IL-12 regulation mediated by p300 and various histone deacetylases （HDACs）. Results presented in this report demonstrated that the transcription factors Ets2 and Spl acted synergistically with p300 to activate the human IL-12 promoter. The histone acetyltransferase （HAT） activity of p300 was required for this synergic effect, because the adenovirus E1A protein inhibited the synergy. Conversely, HDACs repressed the synergic effect of transcription factors and histone acetylation on the activation of IL-12, while p300 was able to rectify it. These data indicated that Ets2 and Spl worked concertedly and synergistically with p300 in the regulation of human IL-12 expression.There has been considerable interest in researching the regulatory mechanisms that control the synthesis of interleukin （IL）-12, which plays a central role in the differentiation of T-helper-1 cells. In this study, we performed a series of transient transfection experiments designed to elucidate the functional relationship between the IL-12 promoter-specific transcription factors （Ets2 and Spl） and histone acetylation modification in IL-12 regulation mediated by p300 and various histone deacetylases （HDACs）. Results presented in this report demonstrated that the transcription factors Ets2 and Spl acted synergistically with p300 to activate the human IL-12 promoter. The histone acetyltransferase （HAT） activity of p300 was required for this synergic effect, because the adenovirus E1A protein inhibited the synergy. Conversely, HDACs repressed the synergic effect of transcription factors and histone acetylation on the activation of IL-12, while p300 was able to rectify it. These data indicated that Ets2 and Spl worked concertedly and synergistically with p300 in the regulation of human IL-12 expression.

关 键 词：Ets2 SP1 histone acetyltransferase P300 INTERLEUKIN-12 

分 类 号：Q51[生物学—生物化学]