活血通脉汤对脑缺血再灌注损伤大鼠血浆TXB_2、6-keto-PGF_(1α)的影响  

Influence of Decoction of Huoxuetongmai on Changes of Plasma TXB_2 and 6-keto-PGF_(1α) Levels During Cerebral Ischemia Reperfusion Injury in Rats

作　　者：杨柳[1] 龚梅芳[1] 

机构地区：[1]武汉大学人民医院中西医结合科,湖北武汉430060

出　　处：《中国微循环》2006年第1期36-38,共3页Journal of Chinese Microcirculation

摘　　要：目的探讨活血通脉汤对脑缺血再灌注损伤大鼠血浆血栓素B2(TXB2)、6-酮前列环素F1α(6-keto-PGF1α)的影响。方法制作脑缺血再灌注模型,70只大鼠随机平均分为活血通脉汤组、阿司匹林阳性对照组、手术组和假手术组,应用放免法分别测定缺血再灌注24、48 h血浆TXB2、6-keto-PGF1α及其比值。结果手术组大鼠脑缺血再灌注24、48h血浆TXB2水平明显高于假手术对照组(P<0.01),6-keto-PGF1α显著低于假手术对照组(P<0.01),血浆TXB2/6-keto-PGF1α比值增加(P<0.01),缺血再灌注24、48 h脑组织缺血范围显著大于假手术对照组(P<0.01)。使用活血通脉汤治疗能降低TXB2水平,升高6-keto-PGF1α水平,使血浆TXB2/6-keto-PGF1α比值保持在正常水平,减少缺血脑组织范围。结论活血通脉汤可纠正脑缺血再灌注后循环血中TXB2/6-keto-PGF1α的平衡失调及降低脑组织缺血程度,减轻脑缺血再灌注损伤。Objective To explore influence of decoction of Huoxuetongmai on plasma TXB2 and 6-keto- PGF1α levels during cerebral ischemia reperfusion injury in rats. Methods 70 rats were randomly divided into four groups, decoction of Huoxuetongmai group（ n = 20）, aspirin group（ n = 20）, CIRI group（ n = 20） and sham operated group（n = 10）. Plasma TXB, and 6-keto-PGF1α levels were measured 24 hours and 48 hours after ischemia reperfusion. Results In CIRI group plasma TXB, concentration obviously increased（P 〈 0.01） at 24 hours and 48 hours of ischemia reperfusion; 6-keto-PGF1α content decreased very significantly at 24 hours and 48 hours of reperfusion（ P 〈 0.01） than the shame operated group; and TXB2/6-keto-PGF1α ratio markedly increased（P 〈0.01）. Decoction of Huoxuetongmai could lower plasma TXB2 levels and elevate plasma 6-ketoPGF1α levels during CIRI, and make the TXB2/6-keto-PGF1α ratio at normal levels. Conclusion Decoction of Huoxuetongmai could alleviate CIRI by correcting TXB2/6-keto-PGF1α imbalance after cerebral ischemia reperfusion injury and reduce the extension of cerebral ischemia at 24 hours and 48 hours after reperfusion.

关 键 词：活血通脉汤 脑缺血再灌注损伤 血栓素B2 前列环素 

分 类 号：R743.33[医药卫生—神经病学与精神病学]