兔VX2肝癌TAE实验方法改良及影像学评价  

Improvements of Transarterial Embolization of Rabbit Hepatic Neoplasmas Model and Imaging Evaluation

作　　者：关键[1] 胡道予[1] 孙振纲[2] 杨国华[2] 罗鸿昌[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院放射科,武汉430030 [2]华中科技大学同济医学院附属同济医院肝脏外科中心,武汉430030 [3]华中科技大学同济医学院附属同济医院超声影像科,武汉430030

出　　处：《临床放射学杂志》2006年第3期277-280,共4页Journal of Clinical Radiology

基　　金：国家"十五"科技攻关项目(2004BA714B)资助

摘　　要：目的改进兔VX2肝癌模型介入治疗的实验方法,分析兔VX2肝肿瘤的影像学表现。材料与方法开腹种植VX2瘤块于兔肝左叶,建立VX2肝肿瘤模型兔15只。2周后CT及彩超证实接种成功。应用显微外科手术临时阻断肝总动脉血流,经胃十二指肠动脉留置导管行术中介入治疗,术后导管埋置于皮下。用多层螺旋CT、彩超和DSA进行兔VX2肝肿瘤的影像学评价。结果行胃十二指肠动脉留置导管成功11只,麻醉过量及术中死亡各1只,另2只因解剖变异未做插管。多层螺旋CT平扫兔VX2肝肿瘤表现为低密度结节灶;彩超上瘤灶为低回声光团,肿瘤周边及内部可见较丰富血流信号;DSA动脉期呈结节状肿瘤染色,以周边染色为主。结论经胃十二指肠动脉留置导管对兔VX2肝癌行术中介入治疗,可进行超选择化疗栓塞,对肝脏血流动力学影响较小,便于术后观察,实验人员完全避免辐射,但对实验条件和实验技术有一定要求。Objective To improve hepatic intra-artefial infusion of rabbit hepatic neoplasmas model and evaluate the image manifestations of the VX2 hepatomas. Materials and Methods 15 New Zealand white rabbits were inoculated with fragment of VX2 carcinoma into the left lobe of the liver. After 2 weeks, all the rabbits were proved with liver neoplasmns by CT scanning and ultrasound. During the open laparotomy, common hepatic artery was ligated temporarily before transarterial catheter embolization （TACE）, and then a catheter was inserted via gastroduodenal artery under microscope and detained in rabbit＇s abdomen. TACE was performed on the rabbits. CT scanning, ultrasound and DSA were performed to describe the image features of rabbit hepatic neoplasmas. Results TACE was successfully done in 11 rabbits. 1 rabbit died of overdosage of anesthesia. 1 rabbits died of massive bleeding during surgery. Artery anatomy variation was existed in 2 rabbits, so catheterization was quitted. VX2 hepatomas were appeared as hypodensity nodules on multiple slice spiral CT, and depicted as low echo, higher blood flow signal on ultresound with rich blood intensity in margin or inner tumor. Trans-gastroduodenal artery angiography showed that VX2 hepatomas had tumor stain （mainly around the tumor）. Conclusion Embolization can be performed superselectively by detained catheter via gastro duodenal artery during the open laparotomy and has little impact on hepatic hemodynamics, it can be easily used to follow up by the detained catheter. Further more, operator can avoid radiation, but the requirements of condition and technology are higher than usual.

关 键 词：兔 肝肿瘤 模型 放射学 介入性 

分 类 号：R735.7[医药卫生—肿瘤] R736.1[医药卫生—临床医学]