不同温度保存对骨髓细胞存活率的影响  

Influence on Preservation Bone Marrow Cell in Different Low Temperature

作　　者：邵同先[1] 邵聃[2] 张苏亚[1] 

机构地区：[1]河南职工医学院,河南郑州450003 [2]西安电子科技大学通信工程学院,陕西西安710071

出　　处：《河南职工医学院学报》2006年第1期64-65,共2页Journal of Henan Medical College For Staff and Workers

摘　　要：目的探讨4℃与-196℃保存骨髓细胞的方法和存活率。方法取wistar大鼠,无菌条件下制备成骨髓细胞悬液,分别于4℃与-196℃保存,保存96 h和60 d后,用Trypanblue染色、观察骨髓细胞的回收率。结果骨髓细胞4℃保存96 h,回收率为28;-196℃保存60 d,细胞回收率为2318。结论-196℃保存骨髓细胞其存活率高于4℃保存,它是中长期保存骨髓细胞的一种有效的方法。Objective To probe into surviving rats of preservation bone marrow cells in 4℃ and - 196 ℃. Methods The authors took bone marrow cells of naked rats and made marrow cells with trypanblue dyeing af- ter preservation 96 h and 60d, Results The retrieve rate of bone marrow cells was 28 in preservation 96 h of 4 ℃. It is 2318 in preservation 60 d of - 196 ℃. Conclusion The retrieve rate of preservation with - 196 ℃ is higher than preservation with 4 ℃ （ P 〈 0.05）. It is an effective preservation method of a middle and long period of time preservation bone marrow cells with - 196 ℃.

关 键 词：低温 保存 骨髓细胞 

分 类 号：R318.52[医药卫生—生物医学工程]