免疫治疗后荷瘤小鼠树突状细胞的功能评价  

Functional Evaluation of Bone Marrow Derived DC of Tumor Bearing Mice After Immunotherapy

作　　者：李敏[1] 陈成[1] 古涛[1] 周桓[1] 张烽[1] 朱一蓓[1] 於葛华[1] 张学光[1] 顾宗江[1] 

机构地区：[1]苏州大学医学生物技术研究所免疫学教研室,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2006年第1期1-3,共3页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30271205);江苏省临床免疫学重点实验室基金资助

摘　　要：目的评价免疫治疗后荷瘤小鼠骨髓来源的树突状细胞(DC)体外激发T细胞增殖及分泌细胞因子的功能。方法采用腹腔注射激发型4-1BB抗体联合DC主动免疫治疗荷瘤小鼠3。H-TdR掺入试验检测免疫治疗后荷瘤小鼠骨髓来源DC体外激发T细胞增殖的能力,ELISA法检测DC刺激T细胞分泌IL-2、IFN-γ及IL-10的水平。结果荷瘤小鼠骨髓前体细胞产生的DC激发T细胞增殖及分泌IL-2、IFN-γ的能力下降,但经过免疫治疗后,这类DC激发T细胞增殖及分泌IL-2、IFN-γ的能力均得到改善,而且免疫治疗效果越好,改善就明显。结论荷瘤小鼠经免疫治疗后,其骨髓前体细胞来源DC的免疫功能得到改善。Objective To evaluate the function of bone marrow derived DC of tumor bearing mice after immunotherapy. Methods Tumor bearing mice were immunized with DC vaccine plus injection of agonistic anti-4-1BB monoclonal antibody. The proliferation of T cells primed with bone marrow derived DC of tumor bearing mice after immunotherapy was tested by ^3H-TdR incorporation. ELISA was employed to detemine the levels of IL-2, IFN-γ and IL-10 secreted by DC primed T cells. Results Bone marrow derived DC of tumor bearing mice was less efficient in stimulating the proliferation of T cells and IL 2 and IFN-γ secretionmade by T cells. After immunotherapy, the proliferation of cells and IL-2 and IFN-γ secretionmade by T cells were enhanced. Conclusion The function of bone marrow derived DC of tumor bearing mice after immunotherapy was ameliorated.

关 键 词：树突状细胞 肿瘤 免疫治疗 

分 类 号：R730.51[医药卫生—肿瘤] Q254[医药卫生—临床医学]