直链藻强化的轮虫对中华绒螯蟹溞状幼体Ⅱ期与Ⅲ期生长及脂肪酸组成的影响  

Effect of Rotifer Enriched by Melosira Granulate on Development and Body Fatty Acids Composition of Eriocheir sinensis Zoeae Larvae

作　　者：王庆[1] 唐晟凯[1] 杨家新[1] 

机构地区：[1]南京师范大学生命科学学院水产研究所,江苏南京210097

出　　处：《南京师大学报（自然科学版）》2006年第1期102-105,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省水产三项更新工程项目(PJ2002-31PJ2003-33)

摘　　要：用直链藻强化培养的轮虫,其EPA和DHA含量显著增加,以强化72 h效果最佳,其EPA和DHA含量分别为10.36%和3.12%;用不同密度的强化轮虫培养中华绒螯蟹溞状幼体Ⅱ期与Ⅲ期(Z2与Z3),结果表明:轮虫密度为20 ind/mL时Z2的变态率与存活率分别为60.60±2.64%和63.20±2.48%,密度为30 ind/mL时Z3的变态率与存活率分别为57.40±1.63%和62.00±2.98%,均为最佳;收集该阶段溞状幼体,对其脂肪酸含量进行分析,强化轮虫培养的溞状幼体的EPA和DHA含量分别为15.09%和7.24%,显著高于未经强化的酵母轮虫培养的溞状幼体(p<0.05).EPA and DHA contents of rotifer increase obviously after enrichment by Melosira granulate. After 72 h enrichment, EPA and DHA contents of rotifer are 10.36% and 3.12% respectively and reach the best effect. The densities of 20 ind/mL and 30 ind/mL of the enriched rotifers are most suitable for the survival and ecdysis of Z2 and Z3 respectively. When the density is 20ind/ml, the ecdysis rate and survival rate of Z2 are 60, 60 ±2.64% and 63.20 ±2. 48% respectively. The ecdysis rate and survival rate of Z3 are 57.40 ± 1.63% and 62.00 ±2. 98% respectively at 30 ind/mL. The body fatty acids composition of Eriocheir sinensis zoeae larvae is evaluated. The results show that the EPA and DHA contents of zoeae larvae fed with enriched rotifer are obviously higher than those fed with unenriched rotifer. The EPA and DHA content reach 15. 09% and 7.24% respectively,

关 键 词：中华绒螫蟹 HUFA EPA DHA 存活率 变态牢 轮虫 

分 类 号：S955.3[农业科学—水产养殖]