SKP2和P27^(Kipl)蛋白在直肠癌中的表达与临床病理特征的相关性研究  

Correlations of SKP2 and P27^(Kipl) protein expressions with clinicopathological features of rectal carcinoma

作　　者：李伟华[1] 柳君[1] 纪宇波[1] 鲁洁琼[1] 付家欣[1] 张兴林[1] 

机构地区：[1]余姚市人民医院,浙江余姚315400

出　　处：《现代实用医学》2006年第2期74-76,83,共4页Modern Practical Medicine

基　　金：宁波市医学科技项目;项目编号2004086。

摘　　要：目的探讨细胞S期激酶相关蛋白(SKP2)和P27K ip l蛋白在直肠癌中的表达及与直肠癌各项临床病理特征的关系,并探讨两者的相关性。方法用免疫组化EnvisionTM法检测62例直肠癌中SKP2和P27K ip l蛋白的表达。结果62例直肠癌组织中P27K ip l、SKP2蛋白的表达率分别为46.77%、33.87%。随肿瘤分化程度降低、浸润深度加深、淋巴结转移和临床分期提高,P27K ip l蛋白表达率逐渐降低(P<0.05);SKP2的表达率随肿瘤分化降低和浸润深度加深而逐渐升高。两者的阳性表达率均与患者的年龄、性别、肿瘤大小无关(P>0.05)。P27K ip l与SKP2表达呈显著负相关(P<0.01)。结论P27K ip l、SKP2的异常表达可加速细胞周期转化,促进直肠癌的发生。对两种蛋白的检测有助于直肠癌恶性程度和预后的判断。Objective To investigate the correlations of P27^Kipl and S-phase kinase-associated protein （SKP2） expressions with the clinicopathohigical features of rectal carcinoma, and the correlation between expressions of SKP2 and P27^Kipl in rectal carcinoma. Methods The expressions of P27^Kipl and SKP2 were determined by EnvisionTM immunohistochemical method in 62 specimens of rectal carcinoma. Results The positive rate of P27^Kipl and SKP2 expression was 46.77% and 33.87% ,respectively. The positive rate of P27^Kipl expression in rectal carcinoma was decreased with the poor differentiation, deep invasion, lymph node metastasis and late TNM stage （P 〈 O. 05 ）, while the positive rate of SKP2 expression was increased with the poor differentiation and deep invasion. The positive rate of both the P27^Kipl and SKP2 expression was not associated with the patient＇s age,sex and tumor size （P 〈 0. 05）. There was a negative correlation between the P27^Kipl and SKP2 expression （P 〈 0.01 ）. Conclusion The abnormal expressions of P27^Kipl and SKP2 may promote cell cycle progression and rectal carcinogenesis. Detecting P27^Kipl and SKP2 protein expressions may be helpful for evaluating the malignant degree and prognosis of rectal carcinoma.

关 键 词：直肠肿瘤 癌症 原癌基因蛋白质类 细胞周期蛋白质类 

分 类 号：R735.37[医药卫生—肿瘤]