蜡蚧轮枝菌昆明菌株(KM9803)的生物学特性研究(二)——培养基种类、储藏条件对菌落生长、产孢量和孢子萌发率的影响  

A Study of Biological Character of Verticillium lecanii KM9803 Strain Isolated from Kunming(Ⅱ)——The Effects of Media Kinds and Preserved Condition on Colonial Growth、Conidiospore Yield and Conidia Germination Rata

作　　者：徐力文[1] 周天雄[1] 杨美林[1] 

机构地区：[1]云南农业大学植物保护学院,云南昆明650201

出　　处：《云南农业大学学报》2006年第2期175-177,共3页Journal of Yunnan Agricultural University

基　　金：教育部重点项目(02143)

摘　　要：用7种培养基培养的结果,在麦芽汁培养基(MEA)上(23℃)得到最大产孢量(4.7×109分生孢子/皿);用6种谷物的固体培养基试验中,大米粒培养基最适宜产孢,发酵培养10 d得到最大产孢量1.5×109分生孢子/g,孢子萌发率95.14%。3种储藏条件试验的结果,以4℃冰箱中黑暗下6个月的孢子萌发率最高(91.47%)。The maximum sporulation yield （4. 7 × 10^9conidia/dish）was obtained on MEA media at 23℃. Experiments of culturing the fungus on six solids grain media showed the rice grain was the most suitable for sporulation yield, as 1.5 ×10^9conidia/g fermented for 10 days. The result of experiments on three kinds preserved condition showed the germination rate of conidiospore was 91.4% in 4℃ refrigerator after 6 months.

关 键 词：培养基 储藏条件 菌落 分生孢子 产孢量 萌发率 

分 类 号：Q935[生物学—微生物学]