荧光动力学法测定抗坏血酸的研究  

Study on the Fluorimetric Kinetic Method for the Determination of Ascorbic Acid

作　　者：陈兰化[1] 李丽花[1] 

机构地区：[1]淮北煤炭师范学院化学系,安徽淮北235000

出　　处：《淮北煤炭师范学院学报（自然科学版）》2006年第1期31-33,共3页Journal of Huaibei Coal Industry Teachers College(Natural Science edition)

摘　　要：在稀硫酸介质中,抗坏血酸能活化钒(V)催化溴酸钾氧化酚藏花红的反应,使其荧光猝灭,建立了荧光动力学法测定抗坏血酸的新方法.测定抗坏血酸的线性范围为2.0～40.0μg.L-1,检出限为1.2μg.L-1.此方法用于药物样品中抗坏血酸含量的测定,加标回收率在97.3%～102.8%之间.A new kinetic-fluorimetric method proposed for determination of ascorbic acid. The method was based on activation effect of ascorbic acid on oxidation of phenosafranine by potassium bromate were catalyzed by vanadium （V） in sulfuric acid medium. The linear range for ascorbic acid was 2.0-40.0μg·L^-1 with detection limit was 1.2μg·L^-1. The method was used to determine ascorbic acid in medicine samples,and recoveries in the range of 97.3%-102.8% were obtained.

关 键 词：抗坏血酸 荧光动力学法 酚藏花红 钒 

分 类 号：O657.3[理学—分析化学]