雄激素替代治疗后去势成年雄性大鼠基底前脑一氧化氮合酶及巢蛋白阳性神经元的变化(英文)  

Changes of the nitric oxide synthase-positive and nestin-positive neurons in the basal forebrain of castrated adult male rats following androgen replacement therapy

作　　者：郭灵[1] 汪华侨[2] 袁群芳[2] 姚志彬[2] 

机构地区：[1]广西医科大学人体解剖学教研室,广西壮族自治区南宁市530021 [2]中山大学中山医学院人体解剖学教研室,广东省广州市510080

出　　处：《中国临床康复》2006年第10期180-183,F0003,共5页Chinese Journal of Clinical Rehabilitation

基　　金：国家教委跨世纪人才基金(国教95-004);广东千百十人才工程基金(97-002)~~

摘　　要：背景:基底前脑的内侧隔核、斜角带核垂直支及水平支的神经元含有丰富的雌激素受体和雄激素受体,故雌、雄激素可通过相应受体作用于神经元,进而影响学习记忆过程。目的:定性和定量分析雄激素替代治疗对成年去势雄性大鼠内侧隔核、斜角带核垂直支及水平支的一氧化氮合酶、巢蛋白阳性神经元的影响。设计:随机对照动物实验。单位:中山大学中山医学院人体解剖学教研室和脑研究室。材料:实验于2001-06/2002-06在中山大学中山医学院人体解剖学教研室和脑研究室完成。选取成年健康雄性SD大鼠28只,随机分为4组(n=7):去势24h后雄激素替代治疗4周组(ART1);去势2周后雄激素替代治疗2周组(ART2);去势24h后载体替代治疗4周组(VRT);假手术组。干预:①ART1组:切除双侧睾丸去势后24h开始皮下注射丙酸睾丸酮(25mg/kg,溶于100μL无菌芝麻油),隔日1次,注射时间固定于上午10:30～11:00,注射14次(4周)。②ART2组:去势后2周开始皮下注射丙酸睾丸酮,剂量和方法同ART1组,注射7次(2周)。③VRT组:去势后24h开始皮下注射无菌芝麻油100μL,注射时间和次数同ART1组。④假手术组:假手术组处理,不伤及睾丸,也不进行任何替代治疗,存活4周。主要观察指标:各组大鼠于相应时间点处死,免疫组织化学法观察内侧隔核、斜角带核垂直支及水平支中的一氧化氮合酶和巢蛋白阳性神经元的形态和数量变化。结果:28只大鼠全部进入结果分析。①各组大鼠各部位的一氧化氮合酶和巢蛋白阳性神经元的形态特征均无明显改变。②一氧化氮合酶和巢蛋白阳性神经元数:在内侧隔核和斜角带核垂直支VRT组大鼠明显高于假手术组(P<0.05或0.01),VRT1和VRT2组明显低于VRT组(P<0.05或0.01),并接近假手术组水平(P>0.05)。结论:雄激素替代治疗对去势成年雄性大鼠基底前脑一氧化氮合酶和巢蛋白阳性神经元的形态无明显影�BACKGROUND： The neurons in the medial septum （MS）, vertical and horizontal limbs of diagonal band of Breca （vDB and hDB） in the basal forebrain contain rich androgen receptors （ARs） and estrogen receptors （ERs） by which androgen and estrogen can act dramatically on the neurons in the basal forebraiu, subsequently affecting learning and memory preceses. OBJECTIVE： To qualitatively anti quantitatively investigate the effects of androgen replacement therapy on the nitric oxide synthase （NOS）-positive and nestin-positive neurons in the MS, vDB and hDB of castrated aduh male rats. DESIGN： A randomly controlled study on experimental animals. SETTING： Department of Anatomy and Brain Research Laboratory of Zhugshan Medical College of Sun Yat-sen University. MATERIALS： The experiment was performed at Depariment of Anatomy and Brain Research Laboratory of Zhongshan Medical College of Sun Yatsen University from June 2001 to June 2002. Totally twenty-eight adult male Sprague-Dawley rats were randomly divided into four groups with seven rats in each group： androgen replacement therapy for 4 weeks following 24 hours of castration （ART1）, androgen replacement therapy for 2 weeks following 2 weeks of castration （ART2）, vehicle replacement therapy for 4 weeks following 24 hours of castration （VRT）, sham-operated group （Sham）. INTERVENTIONS： （1） ARTI group： The castrated rats received subcutaneous injection of testosterone proprionate （25 mg/kg） dissolved in 100 μL of sterile sesame oil every other day from 10：30 am to 11：00 am for 14 times （4 weeks）. （2） ART2 group： The castrated rats received subcutaneous injection of testosterone proprionate with the same dosage and method as ART1 group for 7 times （2 weeks）. （3） The rats in VRT group received subcutaneous injection of 100 μL of sterile sesame oil for 14 times （4 weeks） by the same regime as described above. （4） Rats in Sham group only received sham-operated treatments, and tes

关 键 词：一氧化氮合酶 巢蛋白 雄激素类 基底前脑 睾丸切除术 

分 类 号：R749.053[医药卫生—神经病学与精神病学]