铜绿假单胞菌OXA-1编码基因的克隆及序列分析  

Cloning and Sequencing of Encoding Gene of OXA-1 Subtype of Extended-spectrum β-lactamases in a Pseudomonas Aerugenosa

作　　者：任丽娟 闫志勇[2] 秦东春[3] 王建军[4] 胡军[2] 

机构地区：[1]许昌市中心医院,河南许昌461000 [2]郑州大学基础医学院,河南郑州450052 [3]郑州大学第一附属医院,河南郑州450052 [4]河南科技大学,河南洛阳471003

出　　处：《河南科技大学学报（医学版）》2006年第1期1-4,共4页Journal of Henan University of Science & Technology:Medical Science

基　　金：河南省科技攻关项目(0524410084)

摘　　要：目的研究一株铜绿假单胞菌产生的超广谱β-内酰胺酶(ESBLs)的基因型。方法采用聚合酶链反应(PCR)扩增该株细菌的编码基因片段,克隆入PET-28s载体中,表达于大肠杆菌BL-21感受态细胞,并对PCR产物测序,分析其基因型。结果测序结果显示该株细菌产生的ESBLs为OXA-1亚型,其基因序列与GeneBank中OXA-1编码基因的同源性>99%。结论被研究的铜绿假单胞菌的ESBLs亚型为OXA-1。Objective To investigate the gene type of Extended-spectrum β-1actamases produced by a pseudomonas aerugenosa. Methods The encoding gene of ESBLs produced by the strain was amplified by PCR. The purified PCR products were cloned into PET-28s vector. After they were expressed by BL-21 E. coil cells, the PCR products could be sequenced and analyzed. Results The encoding gene of ESBLs was identified as OXA-1 subtype by PCR and its amino acid sequence was the same as that of OXA-1. Conclusion The ESBLs produced by the Pseudomonas aerugenosa strain is OXA-1.

关 键 词：超广谱Β-内酰胺酶 OXA 序列分析 

分 类 号：R378.991[医药卫生—病原生物学]