分化抑制因子基因在血液系统恶性肿瘤细胞中的表达  

Expression of Id mRNA in malignant tumor cells of hematologic disorders

作　　者：汪萍[1] 李晓军[2] 黄宇烽[2] 贾丽[2] 马百坤[2] 杨家新[1] 

机构地区：[1]南京师范大学生命科学学院,南京210097 [2]南京军区南京总医院全军医学检验中心,南京210002

出　　处：《临床检验杂志》2006年第2期134-136,共3页Chinese Journal of Clinical Laboratory Science

基　　金：江苏省"六大人才高峰"基金课题(编号050203D)

摘　　要：目的研究分化抑制因子(Id)基因在血液系统恶性肿瘤细胞中的表达。方法提取血液病患者及正常人外周血单个核细胞、人Burk itt s B淋巴瘤细胞(Daud i)、人T淋巴细胞性白血病细胞(Jurkat)、人慢性髓性白血病细胞(K562)和人组织细胞性淋巴瘤细胞(U937)的细胞总RNA,根据Id1、Id2和Id3 cDNA全长序列合成三对引物,用RT-PCR方法对以上细胞中Id1、Id2和Id3 mRNA的表达进行半定量分析。结果Id(Id1、Id2、Id3)mRNA在Daud i、U937、Jurkat、K562以及血液病患者的淋巴细胞中均有不同程度的的表达;Id2在正常人淋巴细胞中普遍表达;Id1和Id3几乎不表达或表达水平极弱。结论不同的Id基因在不同分化阶段的淋巴细胞中有不同的表达水平,Id1、Id3的异常表达在淋巴细胞的分化、增殖及肿瘤的发生中可能发挥一定的作用。Objective To study the expression of Id mRNA in hematologic malignant tumor cells. Methods The total cellular RNA were extracted from peripheral blood mononuclear cell （PBMC） of the patients with hematologic disorders and health adults as well as from four tumor cell lines （Daudi,Jurkat, K562, U937）. The primers based on the reported sequences of Id1, Id2 and Id3 were synthesized. The mRNA expression levels of Id1-Id3 in PBMCs and tumor cells were detected by RT-PCR. Results The mRNA expression of Id1 ,Id2 and Id3 showed different levels in different cells. Id2 was widely expressed in both normal and malignant cells as well as many types of lymphocyte. The expression levels of Id1 and Id3 mRNA in tumor cells and PBMC from hematologic disorder patients were significantly higher than those in PBMC of healthy adults. Conclusion Different Id genes have different expression patterns in different cell types. It seems that Id1 and Id3 may play some important roles in the proliferation and differentiation of lymphocytes and in the development and growth of tumors.

关 键 词：分化抑制因子(Id) RT-PCR 淋巴细胞 肿瘤 

分 类 号：R733[医药卫生—肿瘤]