应用A-PAGE电泳方法鉴定豇豆品种  

Application of A-PAGE for Identification of Cowpea Varieties

作　　者：沈晓贤[1] 曹栋栋[1] 胡晋[1] 宋文坚[1] 胡伟民[1] 

机构地区：[1]浙江大学种子科学与工程研究所,杭州310029

出　　处：《种子》2006年第3期19-21,共3页Seed

基　　金：浙江省科技计划项目(2005C32001)

摘　　要：分别用3种提取液提取种子醇溶蛋白,利用改进的ISTA小麦和大麦醇溶蛋白酸性聚丙烯酰胺凝胶电泳(A-PAGE)方法对豇豆不同品种种子进行了鉴定。结果表明,以20%二氯乙醇+1%2-巯基乙醇+18%的尿素为提取液,且加样量为10μl时,电泳图谱条带清晰,可以对豇豆品种进行有效地鉴定。利用超声波处理样品可以缩短样品提取时间并得到同样的提取效果。Different varieties of Vigna unguiculata ssp. sesquipedalis were identified by modified ISTA acid polyacrylamide gel electrophoresis. （A-PAGE）. method with three kinds of solutions for extraction of seed alcohol-soluble proteins. The results showed that the modified PAGE method with alcohol-soluble protein polymorphism bands can be used to verify cuhivar of cowpea when using extraction solution composed of 20% 2-chloroethanol, 1% 2-mercaptothanol and 18% urea and 10 μl loading sample. Ultrasonic treatment could be used to shorten sample extraction time with the same extracting effect.

关 键 词：豇豆 醇溶蛋白 A-PAGE 提取液 超声波 品种鉴定 

分 类 号：S512.102[农业科学—作物学] S643.4