EGCG对中波紫外线诱导HaCaT细胞p53基因和蛋白表达的实验研究  

Effects of EGCG on UVB-induced p53 gene and protein expression in HaCaT keratinocytes

作　　者：苏荣健[1] 金颂良[1] 骆丹[1] 林向飞[1] 闵伟[1] 吉玺[1] 徐丽贤[1] 朱洁[1] 

机构地区：[1]南京医科大学第一附属医院皮肤科,210029

出　　处：《中国麻风皮肤病杂志》2006年第3期187-190,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然基金资助项目(项目批准号30371294)

摘　　要：目的:研究表没食子儿茶素没食子酸酯(EGCG)对中波紫外线(UVB)照射诱导永生化角质形成细胞株-HaCaT细胞的p53 mRNA和p53蛋白表达的影响。方法:以一定剂量UVB照射HaCaT细胞,并以200μg/mL EGCG处理照射后的HaCaT细胞,分别用RT-PCR法和Western blot方法检测各处理条件下p53 mRNA和/或p53蛋白的表达水平。结果:30 mJ/cm2的UVB照射后HaCaT细胞的p53 mR-NA和p53蛋白表达逐渐增加,4 h达到峰值,4 h后随照射剂量增加而增加,24 h后有所恢复;加入EGCG可下调UVB诱导的表达作用。结论:UVB照射对HaCaT细胞p53 mRNA和p53蛋白的诱导表达有时效性与量效性,EGCG可下调UVB照射的这种诱导作用。Objective： To investigate the down regulation effects of epigallocatechin gallate （EGCG） on UVB induced p53 mRNA gene and protein expressions in HaCaT keratinocytes. Methods： HaCaT cells were cultured and irradiated with certain dosage of UVB, and then treated with 200 μg/mL of EGCG. RT - PCR and Western blot were used to detect p53 mRNA and p53 protein expressions. Results： The expressions of both p53 mRNA and p53 protein in HaCaT cells increased gradually and reached a peak at hour 4 and persisted almost for 24 h after the UVB irradiation of 30 mJ/cm^2 in a dose - dependence pattern （ P 〈 0,05 ）. Alter addition of EGCG, the expression of p53 gene and p53 protein was down regulated （ P 〈 0,05 ）, Conclusion： p53 ndtNA and p53 protein expressions of HaCaT cells are time- and dose- dependent on UVB inadiation, and EGCG can down regulate the p53 expression induced by UVB ircadiation.

关 键 词：中波紫外线照射 HACAT细胞 P53 表没食子儿茶素没食子酸酯 

分 类 号：R751[医药卫生—皮肤病学与性病学]