Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization  

作　　者：王全富 缪锦来 侯艳华 郑洲 李光友 

机构地区：[1]Harbin Institute of Technology,School of the Ocean,Weihai 264209,China Key Laboratory of Marine Bio-active Substances,First Institute of Oceanorgarphy,State Oceanic Administration.Qingdao 266061,China [2]Key Laboratory of Marine Bio-active Substances,First Institute of Oceanorgarphy,State Oceanic Administration,Qingdao 266061,China [3]Harbin Institute of Technology,School of the Ocean,Weihai 264209,China

出　　处：《Chinese Journal of Polar Science》2005年第2期81-89,共9页极地研究（英文版）

基　　金：supported by Program of National Natural Science Foundation of China.(No.40406003)

摘　　要：107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases.107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases.

关 键 词：16S rRNA gene psychrophilie bacteria PROTEASE SCREENING Ant-arctic. 

分 类 号：P941.6[天文地球—自然地理学]