大鼠大脑中动脉闭塞后脑内胰岛素样生长因子-1 mRNA和蛋白的表达  

The Expression of Insulin-Like Growth Factor-1 mRNA and Protein After Middle Cerebral Artery Occlusion in Rats

作　　者：高焕民[1] 柳耀泉[1] 王少萍[1] 吕少平[1] 程介士[2] 

机构地区：[1]青岛大学医学院第二附属医院神经科,266042 [2]复旦大学医学院医学神经生物学国家重点实验室,上海200032

出　　处：《国外医学（脑血管疾病分册）》2005年第12期899-902,共4页Foreign Medical Sciences Cerebrovascular Diseases

基　　金：国家自然科学基金项目(编号:30070946)

摘　　要：目的探讨大脑中动脉闭塞(MCAO)后早期胰岛素样生长因子-1(IGF-1)mRNA和蛋白的表达。方法应用12800点DNA芯片、原位杂交和免疫组化方法检测SD大鼠MCAO后IGF-1mRNA和蛋白的表达水平。结果基因芯片结果显示MCAO后缺血侧纹状体内有4480个差异表达基因,其中个别生长因子类基因呈高水平表达,但是多数基因呈低水平表达,IGF-1mRNA在脑缺血后呈相对低水平表达。原位杂交和免疫组化法进一步证实,与假手术组相比,MCAO后缺血侧纹状体内IGF-1mRNA和蛋白阳性细胞数明显减少,再灌注9h和24h时表达均降低(P<0·001)。结论MCAO后早期缺血侧纹状体内IGF-1mRNA和蛋白呈低水平表达,提示IGF-1在脑缺血损伤中可能具有一定的作用。Objective： To explore the expression of insulin-like growth factor-1 （IGF-1） mRNA and protein following middle cerebral artery occlusion （MCAO）. Methods： The 12 800 dots DNA chips analysis, and in situ hybridization and immnuohistochemistry were applied to detect the expression levels of IGF-1 mRNA and protein in SD rats. Results： The result of gene chip showed that 4 480 differential expressed genes in the ischemic striatum following MCAO, among them individual growth factor family genes were up-expressed, whereas most of them were down-expressed. IGE-1 mRNA was down-expressed following cerebral ischemia. In situ hybridization and immunohistochemistry further confirmed that in comparison with the sham-operation group, the numbers of IGF-1 mRNA and protein immuno-positive neurons significantly decreased in the ischemic striatum after MCAO, and the expression decreased after 9 and 24 hours reperfusion （P 〈 0.001 ）. Conclusion： The expression of IGF-1 mRNA and protein in early ischemic striatum following MCAO decreases and this suggests that IGF-1 may play a role in cerebral ischemic injury.

关 键 词：再灌注损伤 胰岛素样生长因子-1 基因芯片 原位杂交 免疫组织化学 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]