低剂量辐射对裸鼠移植人胶质瘤(U251)细胞凋亡相关基因mRNA表达的影响  

Effect of low dose radiation on expression of apoptosis-related gene mRNA of transplantation tumor tissues of human glioma(U251)-bearing nude mice

作　　者：姜宏宇[1] 张国成[1] 王冠军[2] 

机构地区：[1]吉林大学第一医院老年病科,吉林长春130021 [2]吉林大学第一医院血液肿瘤科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2006年第2期301-304,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划资助课题(200505183)

摘　　要：目的:研究低剂量辐射对裸鼠移植人胶质瘤U251细胞凋亡相关基因mRNA表达的影响。方法:35只荷人胶质瘤U251瘤裸小鼠,随机分为7个实验组,假照组:0 mGy;D1组:75 mGy;D2组:4 Gy;D1-12 h+D2组:D1照射后12 h予以D2;D1-24 h+D2组:D1照射后24 h予以D2;D1-48 h+D2组:D1照射后48 h时予以D2;D1-72 h+D2组:D1照射后72 h予以D2。每组5只。各实验组不同方式照射后4 d,荷瘤裸鼠全部处死,采用原位杂交技术检测荷瘤组织的p53、Bcl-2和Bax的mRNA表达水平。结果:D1(75 mGy)和D2(4 Gy)照射后,胶质瘤细胞的p53和Bax的mRNA表达分别呈上升趋势,Bcl-2的mRNA表达呈下降趋势,但与假照组比较,差异无显著性(P>0.05);D1+D2组(D1和D2间隔24、48及72 h)的p53和Bax的mRNA表达明显增多,Bcl-2 mRNA表达显著下降,与假照组比较差异有显著性(P<0.05或P<0.01)。结论:低剂量辐射在一定程度上可能上调了胶质瘤细胞的p53、Bax的mRNA表达,下调了Bcl-2mRNA的表达,同时对其后的大剂量辐射有协同作用。Objective To study the effects of low dose radiation （LDR） on expression of apoptosis-related gene mRNA of transplantation tumor tissues of human glioma （U251） -bearing nude mice. Methods Thirty -five tumor- bearing nude mice were divided into seven groups： sham-irradiated group： 0 mGy; Dl group： 75 mGy; D2 group： 4 Gy; D1-12 h＋D2 group： between Eh and D2 was 12 hi D1-24 h＋D2 group： between D1 and D2 was 24 hi D1-48 h＋D2 group： between D1 and D2 was 48 hi D1-72 h＋ D2 group： between D1 and D2 was 72 h. The expression of apoptosis-related gene mRNA of transplantation tumor tissues of tumor （U251） - bearing nude mice was detected by in situ hybridization. Results There were basal expressions of p53, Bcl-2, Bax mRNA in all groups. The transcription levels of p53 and Bax were slightly up-regulated and Bcl-2 was slightly down-regulated in D1 （75 mGy） group and D2 （4 Gy） group, but there was no significant difference compared with sham-irradiated group （0 mGy） （P〈0. 05） ; While the differences of the levels of p53, Bcl-2, Bax between all D1 ＋D2 groups and sham-irradiated group were significant （P〈0. 05 or P〈0. 01）. Conclusion LDR could up-regulate the transcription level of p53, Bax and down-regulate Bcl-2 to some extent, and it maybe have synergic effect with high dose radiation.

关 键 词：低剂量辐射 人胶质瘤(U251) 裸小鼠 细胞凋亡 基因 原位杂交 

分 类 号：R730.55[医药卫生—肿瘤]