拓扑替康对人卵巢癌顺铂耐药细胞株作用的研究  

The effect of topotecan to cisplation-resistant human ovarian cancer cell lines

作　　者：王世安 艾立清 李箫[2] 毛政[2] 卢运萍[3] 

机构地区：[1]武汉长江航运总医院肿瘤科,湖北430010 [2]华中科技大学同济医学院 [3]附属同济医院肿瘤生物医学中心

出　　处：《中国交通医学杂志》2006年第1期13-14,17,共3页Chinese Medical JOurnal of Communications

基　　金：国家重点基础研究发展规划项目(973)(No:2202CB513100)

摘　　要：目的:探讨拓扑替康(TPT)在体外诱导人卵巢癌顺铂耐药细胞株A2780/DDP凋亡的作用及其对凋亡相关蛋白表达的影响。方法:应用TPT诱导人卵巢癌顺铂耐药细胞株A2780/DDP凋亡,运用MTT法、透射电镜、DNA凝胶电泳检测和观察TPT对人卵巢癌顺铂耐药细胞株A2780/杀伤效应、凋亡形态、生物特性。采用Westernblot印迹杂交法以检测TPT对凋亡相关蛋白bcl-2和bax表达的影响。结果:TPT可诱导细胞株A2780/DDP凋亡,经TPT作用后A2780/DDP细胞内bax蛋白增加,而bcl-2蛋白不表达,bax/bcl-2比值增加。结论:TPT可诱导人卵巢癌顺铂耐药细胞株A2780/DDP凋亡,其机制可能与bax蛋白高表达及bax/bcl-2不表达有关。Objective：To boserve the effect of apoptiosis induced by topotecan（TPT） and study the experssion of apoptosis-related protein in cell lines A2780/DDP.Method：Apopotosis in cultured ovarian cancer cell lines A2780/DDP induced by TPT was investigated in vitro by applying MTr assay,electron microscope,DNA ladder so as to observe kill-efficience and apoptosis cell shape as well as biologhy characteristics.The method of western blot was used to test expression of apoptosis related protein bcl-2 and bax.Results：The cell lines A2780/DDP appeared typical characterisitic of cell apoptosis after being treat by TPT.The bax protein increased while bcl-2 protein expression was unclear.Conclusion：Our study showed that TPT were effective on inducing apoptosis of cisplatin-resistant human ovarian cancer cell lines A2780/DDP.One of the mechanism may be related to the high expression of bax protein and the increase bax/bcl-2 in ratio.

关 键 词：拓扑替康 卵巢癌 细胞凋亡 凋亡相关蛋白 

分 类 号：R730.53[医药卫生—肿瘤]