离体猪眼睫状体上皮电参数测量  

The in Vitro Electrical Parameters of the Isolated Porcine Ciliary Epithelium

作　　者：凌运兰[1] 杜嗣河[2] 林明楷[1] 卓业鸿[1] 侯飞[1] 江志荣[2] 葛坚[1] 

机构地区：[1]眼科学教育部重点实验室中山大学中山眼科中心,广州510060 [2]香港理工大学眼科视光学院视光学实验室

出　　处：《眼科学报》2006年第1期59-62,共4页Eye Science

基　　金：广东省科技计划项目(C30503);卫生部临床学科重点项目(卫规财发[2004]468);广东省自然科学基金(05001713)资助。

摘　　要：目的:测量离体猪眼跨睫状体上皮(ciliary epithelium,CE)电参数,为人眼睫状体研究提供依据。方法:应用改良的Ussing-chamber连续灌注系统对离体的猪眼睫状体上皮电参数:电阻 (resistance,R)、电位差(potential difference,PD)及短路电流(short-circuit current,SCC)进行测量,并观察哇巴因(ouabain)对电参数的影响。结果:猪眼跨睫状体上皮房水侧PD为:(-0．6±0．1)mV,且始终保持负性。SCC及R分别为: (-10．9±1．2)μA／cm2及(54．6±1．6)Ωcm2。0．1mM ouabain应用于房水侧PD及SCC表现为先超极化再去极化,即先是刺激作用,然后是抑制作用。结论:猪眼跨睫状体上皮房水侧PD为负性;Na+,K+-ATP酶存在于猪眼色素上皮(pigmented epithelium,PE)和非色素上皮non-pigmented epithelium,NPE)细胞上,且对维持其睫状体上皮房水侧电极性为负性起重要的作用。Purpose：To study the characteristics of ion transport across the isolated porcine ciliary epithelium to offer the good model for human ciliary epithelium study. Methods ： Fresh isolated pig ciliary epithelium was mounted in a modified Ussing-type chamber to measure the electrical parameters and the effect of 0.1mM ouabain was investigated. Results：The potential difference （PD） across the preparations was （-0.6±0.1） mV and was consistently negative at the aqueous side. The short circuited current （SCC） and resistance （R） were found to be （- 10.9 ± 1.2 ） μA/cm^2 and （54.6 ± 1.6） Ωcm^2 respectively. Both PD and SCC were firstly hyperpolarized （stimulation） and then depolarized when 0. 1mM ouabain was applied to the aqueous side. Conclusion：The polarity of transepithelial PD of the isolated porcine ciliary epithelium was negative at the aqueous side, implying an anion transport may be important in the aqueous humor formation （AHF）. Na^＋, K^＋-ATPase is located on the PE and NPE cells of the porcine ciliary epithelium and plays an important role in AHF.

关 键 词：睫状体上皮 房水 离子转运 猪眼 

分 类 号：R77[医药卫生—眼科]