类胰蛋白酶对微血管内皮细胞中IL-6和TNF-α表达的影响  

Effect of tryptase on the expression of IL-6 and TNF-α in microvascular endothelial cell

作　　者：买买提祖农.买苏尔 马永杰[2] 陆超[2] 赵凤娣[2] 殷莲华[2] 

机构地区：[1]新疆医科大学病理生理学教研室,乌鲁木齐830054 [2]复旦大学上海医学院生理与病理生理学系,上海200032

出　　处：《复旦学报（医学版）》2006年第2期217-221,共5页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金(30470689)资助

摘　　要：目的研究肥大细胞类胰蛋白酶(tryptase)对小鼠脑微血管内皮细胞系bEnd.3表达白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的影响以及可能的信号途径。方法bEnd.3细胞与不同浓度的类胰蛋白酶共同培养后,用RT-PCR和放射免疫的方法检测细胞及其上清液中IL-6、TNF-αmRNA和蛋白的表达量以及蛋白酶激活受体2(PAR-2)对其表达的影响。结果类胰蛋白酶上调bEnd.3IL-6和TNF-αmRNA的表达和分泌,并呈时间和剂量依赖性,这一作用可能是通过激活细胞膜上的PAR-2而发挥的。结论肥大细胞类胰蛋白酶参与bEnd.3微血管内皮细胞的致炎性反应。Purpose To study the effect of mast cell tryptase on the expression of IL-6 and TNF-α in bend. 3 （a mouse brain microvascular endothelial cell strain）. Methods bend. 3 cell strains were cultured with different concentrated tryptase. After isolating total RNA, reverse transcription polymerase chain reaction （RT-PCR） was made to examine the mRNA level of IL-6 and TNF-α in cells. Meanwhile,tryptase-induced cytokine secretion in the cultured supernatants was measured by radioimmunoassay. The effect of PAR-2 on the expression of IL-6 and TNF-α in bend. 3 was also explored. Results Tryptase induced up-regulation of the expression of IL-6, TNF-α mRNA and protein in concentration-dependent and time-dependent manners by activating protease-activated receptor 2 （PAR-2）. Conclusions Mast cell tryptase may be involved in pro-inflammatory reaction in microvascular endothelial cell.

关 键 词：类胰蛋白酶 蛋白酶激活受体2 白细胞介素-6 肿瘤坏死因子-α 炎症 

分 类 号：R363[医药卫生—病理学]