碱性成纤维细胞生长因子对鼠缺血再灌注视网膜神经细胞凋亡及调控基因蛋白表达的干预(英文)  

Intervention of basic fibroblast growth factor on apoptosis of retinal nerve cells and expression of regulatory genes in rats after retinal ischemic reperfusion

作　　者：赵颖[1] 牛膺筠[1] 周占宇[1] 袁春燕[1] 孟岩[1] 

机构地区：[1]青岛大学医学院附属医院眼科,山东省青岛市266003

出　　处：《中国临床康复》2006年第13期181-183,F0003,共4页Chinese Journal of Clinical Rehabilitation

基　　金：山东省教委基金资助(J00K53)~~

摘　　要：背景:碱性成纤维细胞生长因子是一种具有广泛神经活性的多肽生长因子,能保护神经元,促进神经生长。有证据证实碱性成纤维细胞生长因子可治疗视网膜缺血再灌注损伤。目的:建立视网膜缺血再灌注损伤动物模型,分析碱性成纤维细胞生长因子对其视网膜细胞凋亡及调控基因蛋白表达的影响。设计:随机分组,验证性实验。单位:青岛大学医学院附属医院眼科。材料:实验于2002-04/2003-12在青岛大学医学院眼科病理研究室完成。选择健康Wistar大鼠28只,随机取4只作为正常对照组,其余24只大鼠的左眼作为生理盐水对照组,右眼作为碱性成纤维细胞生长因子组。方法:生理盐水对照组、碱性成纤维细胞生长因子组采用前房加压法制作大鼠视网膜缺血再灌注损伤模型。生理盐水对照组从玻璃体腔注入生理盐水12μL,碱性成纤维细胞生长因子组从玻璃体腔注入碱性成纤维细胞生长因子12μL,4只/次。正常对照组不给药。分别于缺血1h再灌注1,6,12,24,48,72h6个时间点采用原位缺口末端标记、免疫组织化学染色检测凋亡细胞的表达,计算凋亡指数。主要观察指标:①各组大鼠再灌注后不同时间点视网膜组织原位凋亡细胞检测结果。②各组大鼠再灌注后不同时间点视网膜组织中Fas、半胱氨酸天冬氨酸蛋白酶-2的表达。结果:①缺血再灌注大鼠不同灌注时间下视网膜组织凋亡指数的比较:正常对照组大鼠视网膜中未见凋亡细胞。与生理盐水对照组比较,碱性成纤维细胞生长因子组于缺血1h再灌注1,6,12,24,48,72h6个时间点凋亡指数均明显降低,且再灌注12,24,48h时差异显著(t=5.362～5.595,P<0.05)。②缺血再灌注大鼠不同灌注时间下Fas表达的变化:正常对照组大鼠视网膜中几乎无Fas表达。与生理盐水对照组比较,碱性成纤维细胞生长因子组于缺血1h再灌注1,6,12,24,48,72h6个时间点Fas表达均明显�BACKGROUND： Basic fibroblast growth factor （bFGF）, a kind of polypeptide growth factor possessing muhifunctional biological activities, can protect neurons and promote the growth of nerves. It has been confinned that bFGF has therapeutic effects on retina isehemia/reperfusion injury （RIRI）. OBJECTIVE： To establish RIRI model and analyze the effects of bFGF on cellular apoptosis Of retina and the expression of regulatory gene protein. DESIGN： Randomized grouping and validating trial. SETTING： Department of Ophthalmology, the Affiliated Hospital of Medical College of Qingdao University. MATERIALS： The experiment was conducted at the Research Laboratory of Pathology, Department of Ophthalmology, Medical College of Qingdao University, from April 2002 to December 2003. Twenty-eight healthy Wistar rats were enrolled in this experiment. Four rats were randomly chosen for normal control group, the left eyes of the other 24 rats were set as normal saline control group, and the right eyes were set as bFGF group. METHODS： Normal saline control group and bFGF group adopted the rat RIRI models established by transiently elevating intraocular pressure. Normal saline of 12 μL was injected into the vitreous cavity of the left eyes of the rats in normal control group.12 μL bFGF was injected into the vitreous cavity of the right eyes of the rats in bFGF group, 4 rats once. No administration was given in normal control group. The expression of apoptotic cells was detected and apoptosis indexes were calculated with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling （TUNEL） method and immunohistochemical staining method at the 1^nt, 6^th, 12^th, 24^th, 48^th and 72^nd hours after reperfusion and isehemia for 1 hour. MAIN OUTCOME MEASURES： ① The detection results of apoptotic cells in situ of retina tissues at different time points after repeffusion. ② The expression of Fas and caspases-2 in retina tissues at different time points after reperfusion. RESULTS �

关 键 词：视网膜 再灌注损伤 成纤维细胞生长因子 碱性 细胞凋亡 

分 类 号：R774.6[医药卫生—眼科]