DAB诱发大白鼠肝癌过程中细胞质膜上酶变化的观察  

OBSERVATION ON THE CHANGES OF ENXYME ACTIVITIES IN PLASMA MEMBRANE FROM RAT LIVERS DURING HEPATOCARCINOGENESIS INDUCED BY DAB

作　　者：黄文心[1] 徐晓利[1] 马涧泉[1] 

机构地区：[1]中山医科大学生物化学教研室

出　　处：《激光生物学》1996年第2期839-844,共6页Acta Laser Biology Sinica

摘　　要：本实验以二甲基氨基偶氮苯（ＤＡＢ）诱发大白鼠肝癌的动物模型为材料，观察了在诱癌过程中和肝癌形成后大白鼠肝细胞质膜上几种酶活性的变化，用不连续蔗糖密度梯度离心法制备肝细胞质膜，分光光度法对酶活性进行定量测定。实验结果表明，在诱无病癌过程中，肝细胞质膜上５′－ＡＭＰａｓｅ活性上降，γ－ＧＴａｓｅ活性显著升高。γ－ＧＴａｓｅ活性升高幅度与病理变化正相关，并且在诱癌早期就能表现出来。We had observed the changeS .of enzymic activities in plasma mambranes duringDAB induced rat hepatocarcinogenesis.The Plamsa membrane of liver cell were taken by discontinuous sucrose density gradient centifugation method. The enzymic actiVities were determined quatitively by spectrophotometry. The results revealed that the 5' -AMPase activities decrease during carcinogenesis,whereas the γ--GTase aetivities showed a great increasewhich were correlated positively with the pathologic change of the hepatic cell.

关 键 词：肝癌 质膜 酶 二甲基 氨基偶氮苯 细胞 

分 类 号：R735.702[医药卫生—肿瘤]