用地高辛标记探针检测基因工程人β干扰素制品中的外源DNA  

The Detection of Residual DNA in Purified rhIFN β with Labelled DIG-DNA

作　　者：白东亭[1] 郑秋君[1] 许丽锋[1] 陈滨[1] 蒋盘宏[1] 

机构地区：[1]北京中国药品生物制品检定所,卫生部北京生物制品研究所

出　　处：《微生物学免疫学进展》1996年第2期46-48,共3页Progress In Microbiology and Immunology

摘　　要：基因工程人β干扰素工程菌经发酵培养、纯化后获得纯的制品，采用斑点杂交技术，用非放射性地高辛标记超声裂解的全工程菌ＤＮＡ作为探针，检测基因工程人β干扰素纯品，结果显示：基因工程人β干扰素纯品中的外源ＤＮＡ含量小于１００ｐｇ／剂。rhIFN-β was expressed in E.coli strain and subseqent purified,the purity of final product was over 95%. Residual DNA in purified rhIFN-β was detected by means of dot blot hydridization,using total DNA of E.coli pBV 320 cells labelled with nonradioactive DIG as a probe,the result showed that residual DNA in final product was far less than 100 pg/dose.

关 键 词：基因工程 人β干扰素 rhIFN-β地高辛 DNA 

分 类 号：R392－33[医药卫生—免疫学]