固相萃取—高效液相色谱法测定藻液中甲磺隆的残留量  

Determination of Metsulfuron-methyl Residues in Algae Water by High Performance Liquid Chromotography with Solid Phase Extraction

作　　者：岳霞丽[1] 张新萍[1] 董元彦[1] 

机构地区：[1]华中农业大学理学院,湖北武汉430070

出　　处：《化学与生物工程》2006年第3期54-55,共2页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(49831005)

摘　　要：建立了固相萃取-高效液相色谱(SPE-HPLC)测定藻液中甲磺隆残留量的方法。实验确定了固相萃取方法,优化了萃取条件。高效液相色谱采用Eclipse XDB-C18(4.6 mm×150 mm,5μm)分离,以甲醇∶水(85∶15,体积比)为流动相,紫外检测波长为227 nm。该方法简便、快速、准确。实验结果表明甲磺隆具有良好的环境特性,将其用于治理“水华”、“赤潮”的急性爆发是可行的。A method has been developed for the determination of metsulfuron-methyl residues in solution of algae by high performance liquid chromotography（HPLC） with solid phase extraction（SPE）. The techniques of SPE including the activation of the SPE column and elution conditions of samples were confirmed. The analytes were separated by using a Eclipse XDB-C18 （4.6 mm×250 mm, 5μm）and methanol and water as mobile phase. The detection wavelength was 227 nm. This method was convenient, rapid, and accurate. The results also showed that metsulfuron-methyl had good environmental behaviors and it could be used to prevent the oceurenee of water bloom and red tide.

关 键 词：固相萃取 高效液相色谱 甲磺隆 残留 

分 类 号：TQ450.1[化学工程—农药化工] X132[环境科学与工程—环境科学]