植物绿叶粗蛋白在SDS-PAGE中向负极泳动的富含苯丙氨酸的碱性蛋白的测定  

The Determination of A Phenylalanine-rich Basic Protein from Plant Green Leaves Migrated towards the Cathode in SDS-PAGE

作　　者：梁秀文[1] 黄丽霞[1] 黄城贵[1] 葛晶[1] 陈红梅[1] 徐杰（1）[1] 董宇亮[1] 郭晋雅[1] 杨净[1] 曾秋莲[1] 徐杰（2） 

机构地区：[1]华南师范大学生命科学学院、广东省植物发育生物工程重点实验室,广州510631

出　　处：《热带亚热带植物学报》2006年第2期126-129,共4页Journal of Tropical and Subtropical Botany

基　　金：广东省自然科学基金(021105)

摘　　要：提取菜心(BrassicacampestrisL.ssp.chinensis(L.)Makinovar.utillisTsenetLee)和生菜(LactucasativaL.)绿叶粗蛋白,经SDS-变性后作醋酸纤维素薄膜电泳,证实这两种蔬菜含有分别向负极泳动和向正极泳动的蛋白。菜心绿叶粗蛋白经SDS-PAGE电泳后,从负极电泳缓冲液中获得向负极泳动的蛋白,其苯丙氨酸含量为57.05%,碱性/酸性氨基酸残基的比例为3.04,表明是富含苯丙氨酸的碱性蛋白。Crude proteins from green leaves ofBrassica campestris L. ssp. chinensis （L.） Makino var. utillis Tsen et Lee and Lactuca sativa L. were subjected to acetate cellulose membrane electrophoresis under SDS-denatured conditions. Two groups of protein were observed, one migrated towards the anode, and the other, the cathode. The cathode protein from Brassica campestris ssp. chinensis vat. utillis was collected from cathode reservoir after SDS-PAGE, and its amino acid components were determined to have 57.0% phenylalanine, the ratio of basic/ acidic amino acid residues was 3.04, indicating the protein towards cathode was a phenylalanine rich basic protein. It must be emphasized that the protein migrated towards the cathode had never been reported previously.

关 键 词：粗蛋白 醋酸纤维薄膜电泳 负极泳动蛋白 SDS-PAGE 苯丙氨酸 

分 类 号：Q946.1[生物学—植物学]