反相高效液相色谱法检测虾青素  

DETERMINATION OF ASTAXANTHIN BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：陈晋明[1] 王世平[1] 陈敏[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院

出　　处：《化学分析计量》2006年第2期27-29,共3页Chemical Analysis And Meterage

基　　金：国家科技攻关计划项目(2001BA804A21)

摘　　要：建立了一种快速检测虾青素的高效液相色谱法。色谱柱为Dikma Dialllonsil^TM-C18（250mm×4．6mmi．d．，5μm），柱温为25℃，甲醇为流动相，检测波长为478nm。虾青素的质量浓度在1～10μg／mL内与色谱峰面积线性关系良好，相关系数为0．9997，该方法测定结果的相对标准偏差为0．38％（n=6），回收率为99．7％。A method for determining astaxanthin by using reversed-phase high performance liquid chromatography was developed. The chromatography conditions were： Dikma Diamonsil^TM-C18 column （250 mm×4.6mm i. d. , 5μm） at 25℃, methanol as mobile phase with the flow rate of 1 mL/min, and detection wavelength at 478 nm. The concentration of astaxanthin was linear with chromatograph peak area in the range of 1-10μg/mL（ r=0.999 7 ）. The RSD was 0.38% （ n=6） , and the recovery was 99.7%. The method was proven to be simple, accurate, and reliable in determining astaxanthin in haematococcus pluvialis and adonis amurensis.

关 键 词：虾青素 高效液相色谱 雨生红球藻 侧金盏花 

分 类 号：O657.72[理学—分析化学] S816.7[理学—化学]