人淋巴瘤白血病细胞系Molt-4的^(31)P核磁共振研究  

^(31)P NUCLEAR MAGNETIC RESONANCE STUDIES OF UMAN LYMPHOMA CELL LINE MOLT-4

作　　者：黄荣清[1] 杜泽涵[2] 杨志刚[3] 冯锐[2] 

机构地区：[1]军事医学科学院放射医学研究所 [2]军事医学科学院国家生物医学分析中心 [3]军事医学科学院基础医学研究所,北京100850

出　　处：《波谱学杂志》1996年第2期107-112,共6页Chinese Journal of Magnetic Resonance

摘　　要：用３１Ｐ核磁共振（ＮＭＲ）观察了人淋巴瘤白血病细胞系Ｍｏｌｔ—４的３１Ｐ谱．ＮＭＲ测试温度为３７℃、２０℃和４℃时．随着测试时间增长，细胞３１Ｐ谱中ＡＴＰ峰降低，无机磷峰升高．细胞３１Ｐ谱的变化随测试温度降低依次减缓．实验温度为４℃，从收集细胞至开始ＮＭＲ累加在３０ｍｉｎ内．ＮＭＲ累加在６０ｍｉｎ内，获得活细胞的稳定的３１Ｐ谱．根据细胞内无机磷的化学位移数值，对细胞内ｐＨ值进行了测定．P nuclear magnetic resonance (NMR) spectra of human lymphoma cell line Molt-4 were observed by using 31P NMR. The decrease in ATP levels and simultaneous increase in inorganic phosphate (Pi) levels occurred as the time of NMR experiment increased under temperatures of 37℃, 20℃ and 4℃.The changes in 31P spectra of cells slow down sequentially following the descent of NMR measurement temperature. Stable 31P spectrum of living cells was obtained when accumulation of data was started within 30min after the harvest of the cells and finished within 60min at 4℃.Intracellular pH of Molt-4 cells was measured based on the chemical shift of Pi peak.

关 键 词：^(31)P核磁共振 MOLT-4细胞 pH 

分 类 号：R733.7[医药卫生—肿瘤] R730.43[医药卫生—临床医学]