γ-谷氨酰转移酶Ⅰ mRNA亚型联合甲胎蛋白mRNA检测对肝癌的诊断价值  

The significance of AFP mRNA and subtypes of GGTⅠmRNA on early diagnosis ofhepatocellular carcinoma

作　　者：沈卫东[1] 黄介飞[1] 魏群[1] 张弘[1] 张喜梅[1] 

机构地区：[1]南通大学附属医院消化内科,南通226001

出　　处：《南通大学学报（医学版）》2006年第2期114-116,118,共4页Journal of Nantong University(Medical sciences)

摘　　要：目的:探讨γ-谷氨酰转移酶ⅠmRNA(GGTⅠmRNA)亚型和甲胎蛋白(AFP)mRNA检测对肝癌的诊断价值。方法:运用RT-PCR检测肝脏组织标本和人肝细胞株QSG-7701、人肝癌细胞株HepG2中GGTⅠmRNA A、B、C3种亚型和AFP mRNA的表达。结果:AFP mRNA在肝癌组织中的表达显著高于非癌肝组织、癌旁组织和远癌组织。GGTⅠmRNA A亚型主要在非癌肝组织中表达,B亚型主要在远癌组织、癌旁组织、癌组织中表达。人肝细胞株QSG-7701中GGTⅠmRNA C亚型阳性表达,人肝癌细胞株HepG2中AFP mRNA、GGTⅠmRNA B亚型表达阳性。GGTⅠmRNA B亚型联合AFP mRNA诊断肝癌的阳性率可达98.0%,小肝癌的阳性率达95.2%。结论:在肝脏癌变的过程中存在着GGTⅠmRNA A亚型向B亚型的转化,联合监测GGTⅠmRNA亚型和AFP mRNA有助于对肝癌的早期诊断。Objective：To study the expression of GGT I mRNA and AFP mRNA in hepatoceUular carcinoma（PHCC） and explore the significance of their expression in the process of PHCC. Methods： GGT I mRNA type A , B , C and AFP mRNA of liver tissues, QSG - 7701 and HepG2 cell line were checked by RT - PCR. Results ：The positive rate of AFP mRNA in liver cancerous tissues was higher significantly than in liver tissues without PHCC, adjacent liver cancerous tissues, and distal liver cancerous tissues. GGT Ⅰ mRNA type A was mainly expressed in livers without PHCC, type B were mainly found in liver cancerous tissues, adjacent liver cancerous tissues, and distal liver cancerous tissues. GGT Ⅰ mRNA type C was expressed in QSG - 7701 cell line, type B and AFP mRNA were expressed in HepG2 cell line. The positive rate of GGT Ⅰ mRNA type B or AFP mRNA were 98.0% in liver cancerous tissues and 95.2% in small liver cancerous tissues. Conclusion：The GGT I mRNA expression in human liver will shift from type A to type B during the development of PHCC. The AFP mRNA and the fragment analysis of GGT I mRNA may be a sensitive assay to diagnose PHCC.

关 键 词：发性肝细胞癌 Γ-谷氨酰转移酶 甲胎蛋白类 宴时荧光-聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]