大黄素、生长抑素对急性胰腺炎大鼠肠黏膜细胞的保护机制  

Role of emodin and somatostatin in protection of intestinal mucosal barrier cells in rats with acute pancreatitis

作　　者：吴薇[1] 张嘉[1] 刘牧林[1] 程兴望[1] 

机构地区：[1]蚌埠医学院附属医院胃肠外科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2006年第2期124-126,共3页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究资助项目(2003kj265)

摘　　要：目的:探讨急性胰腺炎大鼠肠黏膜细胞膜的流动性和细胞膜“钙泵”活性的变化机制,并研究大黄素和生长抑素对上述变化影响。方法:雄性SD大鼠80只,随机分为假手术组、生理盐水处理组、大黄素处理组、生长抑素处理组,5%牛磺胆酸钠逆行性胆胰管注射制作急性胰腺炎大鼠模型,制模6 h后测定大鼠回肠组织细胞膜钙泵活性、荧光偏振度值、丙二醛含量及回肠组织黏膜血流量。结果:与生理盐水处理组相比较,大黄素处理组和生长抑素处理组的丙二醛含量降低(P<0.01),细胞膜钙泵活性、细胞膜流动性增加(P<0.01),肠黏膜缺血得到显著改善。结论:大黄素和生长抑素可通过改善肠黏膜缺血、提高钙泵活性并改善肠黏膜细胞膜流动性,从而起到保护肠黏膜屏障的作用。Objective：To explore the alterative mechanism of intestinal mucosal cell membrane fluidity and calcium pump in rats with acute pancreatitis（AP）, and to study the effects of emodin and somatostatin on them. Methods： Eighty male SD rats were randomly divided into four groups：sham operation group （SO） , normal saline（NS） group, emodin group and somatostalin group. Intestinal mucosal cell membrane Ca^2＋-Mg^2＋ ATPase activity, fluorescence polarization, malondialdehyde （ MDA ） content and blood flow of intestinal mucosa were determined 6 hours after the model rats of acute pancreatitis were established. Results： In the emodin group and somatostatin group,the MDA content was lower than that in the SO group（ P〈0.01 ） ,Ca^2＋-Mg^2＋. ATPase activity and membrane fluidity were higher than those in SO group（P〈0.01） and the decreased value of blood flow of intestinal mucosa was lower compared with that of SO group. Conclusions： Emodin and somatostatin can protect the function of intestinal mucosal barrier by ameliorating intestinal mucosa ischemia,improving calcium pump activity and elevating intestinal mucosal cell membrane fluidity.

关 键 词：胰腺炎 大黄素 生长抑素 肠黏膜屏障 大鼠 

分 类 号：R576[医药卫生—消化系统]