Harpin_(Ea)激发与烟草品种可溶性蛋白质变化的关系  

Changes of Soluble Proteins in Tobaccos Excited by Harpin_(Ea)

作　　者：乔卿梅[1] 程茂高[1] 王素琴[2] 蒋士君[3] 王鹏[1] 

机构地区：[1]郑州牧业工程高等专科学校,郑州市金水区北林路16号450011 [2]河南省农科院烟草研究所,河南省许昌市北郊461000 [3]河南农业大学,郑州市文化路95号450002

出　　处：《烟草科技》2006年第3期30-32,共3页Tobacco Science & Technology

基　　金：河南省教育厅科技攻关项目(200130465)"烟草品种抗病性快速鉴定技术研究"的一部分。

摘　　要：为研究烟草的应激反应,以HarpinEa作激发子,对烟草品种G140、红花大金元(红大)、NC89、G28、K326、和许金2号进行了激发试验,并对激发前后的可溶性蛋白质进行了SDS-聚丙烯酰氨凝胶电泳分析和凝胶成像分析。结果显示HarpinEa激发后,所有供试烟草品种的可溶性蛋白质均发生了变化,其中一些可溶性蛋白质含量明显提高,一些可溶性蛋白质含量降低,同时一些品种还合成了新的可溶性蛋白质。To research the response of tobacco to excitation, the experiments on tobacco cultivars, G140, Hongda, NC89, G28, K326, and Xujin2, were conducted with HarpinEa as the exciton. 30ttg/mL aque- ous HarpinEa solution was injected into tobacco leaves to excite their hypersensitive response, then the soluble proteins in tobacco before and after excitation were analyzed by SDS-PAGE and scanning analysed with a gel imaging analysis system. The results showed that the contents of soluble proteins in all treated tobacco cultivars were changed, some increased significantly and some reduced, besides, there were new soluble proteins composed in some cultivars.

关 键 词：HarpinEa 烟草 品种 可溶性蛋白质 

分 类 号：TS413[农业科学—烟草工业]