头颈鳞癌患者血清6种细胞因子含量与临床相关性分析  

Analysis of the Co-relationship Between 6 Kinds Cytokines Level in Serum and Cancer Staging in Patients with Head and Neck Squamous Cell Carcinoma

作　　者：汤諹[1] 刘流[1] 韦嘉[2] 肖旭辉[1] 杨湛[1] 李志军[1] 谢宏琼[2] 陈红英[2] 

机构地区：[1]昆明医学院第一附属医院口腔颌面外科 [2]昆明医学院第一附属医院感染疾病科

出　　处：《口腔颌面外科杂志》2006年第1期27-31,共5页Journal of Oral and Maxillofacial Surgery

基　　金：云南省教育厅资助项目(04Z028C);昆明医学院第一附属医院科研基金资助项目(2005KJT04)

摘　　要：目的:了解头颈鳞癌患者血清中IL-4I、L-10I、L-12I、L-18I、NF-γ、TGF-β细胞因子的水平及其与患者病情的相关性,探讨6种细胞因子在头颈鳞癌患者疾病发生、发展过程中的变化规律。方法:收集2003-2004年经病理诊断确诊的26例头颈鳞癌患者的空腹血清标本,用双抗体夹心法定量检测血清中IL-4I、L-10I、L-12I、L-18I、NF-γ、TGF-β水平;用流式细胞术定量分析外周血中CD4+/CD8+绝对数量和相对比值,以22例健康成人为对照组。结果采用非参数检验T检验以及非参数秩相关分析进行统计学处理。结果:血清中IL-12I、L-10I、L-4I、FN-γ在实验组和对照组均低于最低检出水平而未检出;头颈鳞癌患者的血清IL-18水平高于正常对照组,且临床分期越晚,病情越重,IL-18水平越高;头颈鳞癌患者组与对照组血清TGF-β1水平无显著性差异;经流式细胞仪检测,头颈鳞癌患者CD3+T细胞总数,CD4+/CD8+比值,NK细胞数量均在正常范围内。结论:血清IL-18水平可能成为头颈部鳞癌诊断和疗效评估的标志物,目前对于头颈部鳞癌患者血清IL-18水平升高途径及其作用机制尚不清楚,进一步了解免疫学途径或许能为头颈部鳞癌患者应用免疫治疗策略提供新的选择。Objective： To explore the co-relationship between serum level of 6 kinds cytokines and HNSCC clinical features in patients with head and neck squamous cell cancer（HNSCC）. Methods： 26 cases patients with HNSCC had been diagnosed by histopathologic methods. Sandwich enzyme-linked immunoabsorbent assays （ sandwich ELISA） commercial kit quantitative analysis were used to determine the serum level of IL-12, IL-4, IL-10, IFN-% IL-18 and TGF-β1 in these patients and 22 healthy controls. CD3^＋,CD4＋/CD8^＋ T lymphocyte, natural killer cell（NK） in peripheral blood were counted by flow cytometry （detected by BDFACS Calibur-E4401）. Results were statistically analyzed by nonparametric tests for test of two independent sample, nonparametric co-relation test and independent-Sample T test. Results： The serum levels of IL-12, IL-10, IL-4 and IFN-γ both in HNSCC and healthy controls were under the detectable level and not detected. There were not significant different between HNSCC groups and healthy controls in serum levels of TGF-β1. The counting of CD3^＋, CD4^＋, CD8^＋T lymphocyte, NK in peripheral blood both in patients and controls groups are in normal range. However, the serum level of IL-18 in HNSCC group was significant higher than that of healthy controls. Furthermore, serum levels of IL-18 in HNSCC group, had co-relation with HNSCC patients＇ cancer staging, The higher level of IL-18, the worse condition of HNSCC patients. Conclusion： The results suggested that IL-18 level in serum might be a candidate for a prognosis or therapeutic responses marker of HNSCC. The pathways for IL-18 production and its mechanisms of action in patients with HNSCC remain to be determined. Understanding of the immunological pathways might o ffer new therapeutic options in head and neck cancer in the future.

关 键 词：头颈部 鳞状细胞癌 辅助T淋巴细胞 细胞因子 

分 类 号：R739.91[医药卫生—肿瘤]