大黄提取物对犬肢体枪弹伤后脑神经元c-Jun蛋白表达变化的影响  

Effect of rhubarb extract on changes of c-Jun expression in cerebral neurons of canines with gunshot wounds in the limbs

作　　者：封亚平[1] 章翔[2] 刘艳生[1] 朱辉[1] 封雨[1] 王典春[1] 

机构地区：[1]解放军成都军区昆明总医院神经外科,云南省昆明市650032 [2]解放军第四军医大学西京医院全军神经外科研究所,陕西省西安市710032

出　　处：《中国临床康复》2006年第11期50-52,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察中药大黄治疗后肢体高能量枪弹伤犬脑神经元c-Jun蛋白表达的变化,从分子生物水平上探讨中药大黄治疗肢体火器伤后远达效应的脑保护途径。方法:实验于2001-07/12在解放军第四军医大学西京医院实验外科完成。18只杂种犬,以M-193,5.56mm制式弹射击犬双后肢肌肉丰满处,随机分为对照组、大黄治疗组,每组分伤后2,6,10h3个时间点,每个时间点3只。对照组伤后应用无菌注射用水鼻饲,大黄治疗组应用中药大黄的乙醇及水提液鼻饲,采用免疫组化方法观察两组伤后2,6,10h远隔部位脑组织脑神经元中c-Jun蛋白的表达变化。结果:18只杂种犬均进入结果分析。伤后2,6,10h大黄治疗组脑神经元中c-Jun蛋白显著少于对照组[(8.7±1.2),(14.2±1.7)个/单位面积;(3.7±2.5),(21.6±2.1)个/单位面积;(11.6±1.4),(19.2±1.9)个/单位面积,P<0.01]。结论:中药大黄提取液能够抑制肢体枪弹伤后脑神经元c-Jun蛋白的表达。AIM： To observe the effeet of rhubarb on changes of c-Jun protein in cerebral neurons of canines with gunshot wounds in the limbs and investigate protective pathway of brain on the hasis of molecular biology. METHODS： The experiment was completed in the Experimental Surgery of Xijing Hospital of the Fourth Military Medical University of Chinese PLA from July to December 2001, Eighteen canines were divided randomly into control groups and rhubarb therapeutic groups, and each group was redivided into three time points including 2 hours, 6 hours and 10 hours with 3 anilnals in each subgroup. M-193, 5.56 mm bullets were used to shoot the most plentiful part of the hind extremity of the canines. Canines in control groups received sterilized waler by nasal feeding post-injury, and alcohol and water solutions of rhubarb extracts by nasal feeding post-injury in rhubarb therapeutic groups. Expressions c-Jun protein in neurons of cerebral tissues at 2 hours, 6 hours and 12 hours after gunshot in two groups were detected with immunohistochemistry. RESULTS： Totally 18 canines entered the final analysis. Numbers of c- Jun protein in cerebral neurons at 2 hours, 6 hours and 12 hours after gunshot was lower in the .rhubarb therapeutic group than those in the control group [（8.7±1.2）, （14.2±1.7）/unit area; （3.7±2.5）, （21.6±2.1）/unit area： （11.6± 1.4）, （ 19.2± 1.9）/unit area, P 〈 0.01 ]. CONCLUSION： Rhubarb extracts can inhibit expression of c-Jun protein in cerebral neurons of canines with gunshot wounds in the limbs.

关 键 词：脑损伤 火器 原癌基因蛋白质c-jun 大黄 免疫组织化学 

分 类 号：R743[医药卫生—神经病学与精神病学]