黄角颗粒对大脑中动脉栓塞老年大鼠脑神经细胞凋亡形态学的影响(英文)  

Morphologic effect of huangjiao granule on apoptosis of cerebral neurocytes in elderly rats with middle cerebral arterial occlusion

作　　者：陈国华[1] 单萍[1] 吴家顺[1] 

机构地区：[1]华中科技大学同济医学院附属中西医结合医院(武汉市第一医院)神经内科,湖北省武汉市430022

出　　处：《中国临床康复》2006年第11期182-185,F0003,共5页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:黄角颗粒为纯中药制剂,具有通腑泄热、开窍熄风、活血化淤等功效。目的:观察黄角颗粒对大鼠大脑中动脉栓塞模型神经细胞凋亡的影响,探讨黄角颗粒治疗急性期脑梗死的可能机制。设计:完全随机分组设计,对照动物实验。单位:华中科技大学同济医学院附属中西医结合医院神经内科。材料:实验于2003-09/2003-12在湖北中医学院中心实验室完成。采用鼠龄为22个月的老年Wistar大鼠60只。大鼠适应性喂养3d后,按体质量随机分为6组:正常对照组、模型组、尼莫地平组、黄角颗粒10g/kg组、黄角颗粒5g/kg组、黄角颗粒2.5g/kg组,每组10只。方法:①采用大脑中动脉栓塞法将大鼠造成脑缺血模型。黄角颗粒(主要成分为生大黄、水牛角等,武汉市第一医院制剂室提供,批号:021120)10,5,2.5g/kg组于造模前30min分别给予含生药1,0.5,0.25kg/L的黄角颗粒水溶液按10,5,2.5g/kg灌胃,每只灌胃1次;尼莫地平组于造模前30min给予含生药1g/L的尼莫地平水溶液按10mg/kg灌胃,每只灌胃1次;模型组和正常对照组(只结扎颈外动脉)于造模前30min给予生理盐水按10mL/kg灌胃,每只灌胃1次。②采用光学显微镜观察原位细胞凋亡染色切片,在高倍镜(20×10)下观察5个视野,观察每个高倍视野平均阳性细胞数,并计算细胞凋亡率。普通光镜下观察皮质缺血局部及周围组织学变化。电镜下观察大鼠大脑皮质细胞超微结构变化。③计量资料差异比较采用F检验及Q检验。主要观察指标:黄角颗粒对大脑中动脉栓塞老年大鼠脑组织神经细胞凋亡率及大脑皮质细胞形态学的影响。结果:Wistar大鼠60只均进入结果分析。①模型组大鼠脑组织凋亡阳性细胞数及细胞凋亡率明显高于正常对照组(P<0.01);黄角颗粒各剂量组及尼莫地平组大鼠脑组织凋亡阳性细胞数及细胞凋亡率明显低于模型组(P<0.01);黄角颗粒10g/kg组大鼠脑组织凋亡阳BAUKGROUND： Huangjiao granule is pure Chinese drugs pharmaceuties, which can eliminate heat by purgatingfu-organs, clear unconsciousness and stop endogenous wind as well as promote blood flow and remove blood stasis etc. OBJECTIVE： To observe the effect of huangjiao granule on apoptosis of neuroeytes in rat models with middle cerebral arterial occlusion, and probe into the possible mechanism of huangjiao granule in the treatment of acute cerebral infarction. DESIGN： Complete randomized grouping design, control animal experiment. SETTING： Department of Neurology, Affiliated Hospital of Traditional Chinese Medicine Combined with Western Medicine, Tongji Medical College of Huazhong University of Science and Technology. MATERIALS： The experiment was conducted in the Central Laboratory, Hubei College of Traditional Chinese Medicine. Sixty old Wistar rats aged 22 months were selected. After 3-day-feeding for adaptability, rats were randomized into 6 groups according to body mass： Normal control group, model group, nimodipine gorup, 10 g/kg huangjiao granule group and 5 g/kg huangj/ao granule group with 10 rats in each group. METHODS： ①Rats were made into cerebral ischemic models with middle cerebral arterial occlusion method. Thirty minutes before modeling, rats in the 10, 5, 2.5 g/kg huangjiao granule （main gredients were radix et rhizoma rhei, cornu bubali etc. provided by Manufacturing Laboratory of Wuhan First Hospital with the batch number of 021120） groups respectively received gastric perfusion of 1, 0.5, 0.25 g/mL huangjiao water solution containing crude drug at the dose of 10, 5, 2.5 g/kg with each rats perfused once; Rats in the nimodipine group received gastric perfusion of 1 mg/mL nimodipine water solution, containing crude drug at the dose of 10 mg/kg with each rats perfused once; Rats in the model group and normal control group received gastric perfusion of normal saline at the dose of 10 mL/kg with each rats perfused once. ② Apoptotic stainning sections in situ were obser

关 键 词：脑梗塞/中药疗法 细胞凋亡 疾病模型 动物 

分 类 号：R743[医药卫生—神经病学与精神病学]