四个马铃薯品种幼茎段再生技术的研究  

Study on in vitro regeneration technology from stem explants of four potato cultivars

作　　者：李娟[1] 程智慧[1] 张国裕[1] 

机构地区：[1]西北农林科技大学 园艺学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2006年第3期122-126,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：中德政府间合作项目(GH-01/04);中德农业部科技合作项目

摘　　要：以4个马铃薯栽培品种为试材,进行了其茎段的离体再生研究。结果表明,东农303、夏波帝、鄂1 号茎段愈伤组织最佳诱导培养基为MS+6-BA 3．0 mg／L+NAA 0．01 mg／L;费乌瑞它为MS+6-BA 3．0 mg／L+ NAA 0．1mg／L,愈伤组织的诱导率均可达100％。费乌瑞它和鄂1号不定芽分化诱导的最佳培养基均为MS+ 6-BA 2．0 mg／L+NAA 0．2 mg／L;东农303为MS+6-BA 2．0 mg／L+NAA 0．1 mg／L:夏波帝为MS+6-BA 2．0 mg／L+NAA 0．3 mg／L,其不定芽分化率分别达96．7％．96．7％,83．3％和80．0％。各品种不定芽的诱导生根率均为100％,试管苗移栽成活率为95％。Four potato cultivars were regenerated in vitro from stem explants. The results showed that the optimal medium for callus induction was found to be MS＋6 -BA 3.0 mg/L ＋NAA 0.01 mg/L for cv. Dongnong 303,cv. Shepody and cv. E No. 1,MS＋6-BA 3.0 mg/L＋NAA 0. 1 mg/L for cv. Favorite. The callus induction rate on those media reached up to 100%. The optimal medium for adventitious bud differentiation from stem callus was found to be MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L for cv. Favorite and cv. E No. 1,MS＋6-BA 2.0 mg/L＋NAA 0. 1 mg/L for cv. Dongnong 303,and MS＋6-BA 2.0 mg/L＋NAA 0.3 mg/L for cv. Shepody. The adventitious bud differentiation rate reached 96.7%, 96.7%,83.3% and 80.0% for the four cultivars respectively,and the rooting rate was 100% for all cultivars. The survival rate of tube plantlets after transplantation reached 95%.

关 键 词：马铃薯 茎段 愈伤组织 植株再生 

分 类 号：S532.035.3[农业科学—作物学]