儿童急性呼吸道博卡病毒感染  

Human Bocavirus Infection among the Hospitalized Children with Acute Respiratory Tract Disease in China

作　　者：瞿小旺[1] 漆正宇[1] 段招军[1] 刘劲松[2] 刘巧突[2] 刘文培[1] 黄灿平[3] 谢志萍[1] 彭夫望[1] 高寒春[3] 郑丽舒[1] 侯云德[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]湖南省郴州市第一人民医院,湖南郴州423000 [3]病毒生物技术国家工程研究中心,北京100076

出　　处：《病毒学报》2006年第2期79-82,共4页Chinese Journal of Virology

摘　　要：了解博卡病毒（Human Bocavirus，HBoV）在我国儿童急性呼吸道疾病中的感染情况。采用PCR扩增的方法对2005年10月～2006年1月收集的72例急性呼吸道感染的住院儿童鼻咽抽吸物（nasopharyngeal aspirates，NPA）进行了HBoV基因检测。将PCR阳性产物进行TA克隆，测序，并将所测序列与GenBank中HBoV序列进行比较分析。72份标本中共检测到6份HBoV阳性扩增产物，阳性率为8．3％（6／72），该6例HBoV刚性患儿临床均有肺炎或支气管肺炎症状。由此可以初步看出HBoV可能也是儿童急性呼吸道感染中较为重要的一个病原，且可能与儿童急性下呼吸道感染存在相关性。In order to understand the epidemic of human boeavirus （HBoV） in China, PCR assay was applied to investigate HBoV infection among hospitalized children with acute respiratory tract disease （ARTI） from October 15, 2005 to January 8, 2006.Samples of nasopharyngeal aspirates（NPA） were collected and nucleic acids were extracted. Six of 72 samples（8.3 % ） were tested positive for HBoV by PCR. The PCR products were separately linked with T-vector and cloned, sequenced. Six gene sequences were compared and analyzed with those in GenBank. All these HBoV- infected patients had clinical symptoms of pneumonia. From above, we can conclude tentatively that HBoV may be an important pathogen in children with ARTI and probably has a correlation with acute lower respiratory tract infection of the children. This is the first report on HBoV in China.

关 键 词：急性呼吸道感染 细小病毒B19 博卡病毒 

分 类 号：R373.1[医药卫生—病原生物学] R392[医药卫生—基础医学]