毛细管电泳-安培法测定酵母菌中的RNA  

Determination of Content of RNA in Yeast by Capillary Zone Electrophoresis with Amperometric Detection

作　　者：陈相[1] 王清江[1] 丁飞[1] 吕金[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《华东师范大学学报（自然科学版）》2006年第2期7-12,共6页Journal of East China Normal University(Natural Science)

基　　金：上海市科委自然科学基础研究基金(04ZR14041)

摘　　要：报道了通过毛细管电泳-安培检测法测定腺嘌呤(A)和鸟嘌呤(G)来间接测定酵母菌中核糖核酸含量的方法.探讨了电极电位、运行缓冲溶液、分离电压及进样时间等对毛细管电泳分离-安培检测的影响,得到了较为优化的测定条件.在30 mmol/L的硼酸盐缓冲液(pH 9.0)中,上述两组分可在18 min内完全分离.腺嘌呤和鸟嘌呤的检测限分别为2.0×10-7mol/L和1.7×10-7mol/L.该法具有较好的重现性和灵敏度,可以用于食品中酵母菌中核糖核酸的快速测定.The indirect determination of the content of RNA in yeast by analyzing adenine （A） and guanine （G） with capillary electrophoresis with amperometric detection （CE-AD） was repor ted. The effects of potential of working electrode, running buffer, separation voltage and injection time on the CE-AD determination were investigated. Adenine and guanine could be well separated in a 30 mmol/L horate buffer （pH 9. 0） within 18 rain under the optimum conditions. Their detection limits of adenine （S/N=3） of 2.0×10^-7mol/L and 1.7×10^-7mol/L, respectively. The experimental results showed that this method is of high sensitivity and high repeatability, and potential to be used in the quick analysis of yeast in foods.

关 键 词：毛细管电泳 安培检测 酵母菌 核糖核酸 

分 类 号：O657[理学—分析化学]