肝细胞移植治疗失代偿期肝硬化的实验研究  

Effect of HCT on rats with decompensated cirrhosis and influence of HGF on hepatocytes transplanted

作　　者：吴颖[1] 张志坚[2] 

机构地区：[1]第一军医大学,广东广州510025 [2]南京军区福州总医院

出　　处：《山东医药》2006年第8期14-16,共3页Shandong Medical Journal

基　　金：福建省青年科技人才创新项目(2002J059)

摘　　要：目的探讨肝细胞移植(HCT)对失代偿期肝硬化鼠的治疗作用和肝细胞生长因子(HGF)对移植肝细胞的影响。方法将70只失代偿期肝硬化大鼠分为3组,治疗1组(T 1组,30只),脾内移植肝细胞2m l(2.0×107个/m l);治疗2组(T 2组,30只),在T 1组基础上加尾静脉注射HGF(5m g/100g);对照组(P组,10只)尾静脉注射生理盐水2m l。HCT后第4周计算存活率,第1、2、4、8、12周检测各组丙氨酸转氨酶(ALT)、总胆红素(TSB)、白蛋白(ALB)、凝血酶原时间(PT)和脾内移植肝细胞的凋亡指数(A I)。结果HCT后第4周,P组的存活率显著低于T 1、T 2组(P均<0.05);从第1周起P组ALT、TSB、ALB水平、第2周起PT值显著高于T 1、T 2组,P均<0.05。T 1组ALT、ALB水平第4周起、TSB、PT值第8周起显著高于T 2组,P均<0.05;T 2组脾内移植肝细胞的A I显著低于T 1组(P<0.05)。结论HCT可明显改善失代偿期肝硬化鼠的肝功能并延长其生存期;HGF能减少移植肝细胞的凋亡。Objective： To study effect of hepatocyte transplantation （HCT） on rats with decompensated cirrhosis and influence of hepatocyte growth factor （HGF） on hepatocytes transplanted. Methods： Seventy rats with decompensated cirrhosis were divided into three groups. No. 1 HCT group （T1） were given 2ml（2. 0 × 10^7/ ml）liver cells into spleen, while No. 2 group （T2） accepted the same amount of hepatocytes and additional HGF （5mg/100g） by caudal vein,but the control group（P） were given 2ml of normal saline only. Survival rates were calculated at the forth week after HCT and ALT, TSB, ALB, PT and apoptosis index （AI） of hepatocytes transplanted were detected at the 1st, 2nd, 4th, 8th. 12th week also after HCT. Results： The survival rate of the 4th week after HCT of P group were significantly lower than those of T1 and T2 groups （P〈0. 05）. The those of ALT, TSB, ALB since the 1st week and PT the value since the 2nd week after HCT in P group were higher than those in T1 and T2 groups obviously （P〈0.05）. The levels of ALT, ALB since the 4th week and TSB, PT since the 8th week after HCT in T1 group were significantly higher than those in T2 group （P〈 0. 05）. Apoptosis index of transphnic transplanted hepatocytes in T2 group was significantly lower than that in T1 group （P〈0. 05）. Conclusions： Liver function is improved obviously and survival periods prolonged in rats with decompensated cirrhosis by HCT. Apoptosis of hepatocytes transplanted maybe decrease by using of HGF.

关 键 词：肝硬化 大鼠 肝移植 肝功能 细胞凋亡 

分 类 号：R575.2[医药卫生—消化系统]