针刺对金黄地鼠视交叉上核c-Fos表达的影响  

Effect of Electroacupuncture on the Expression of c-Fos in the Syrian Golden Hamster Suprachiasmatic Nucleus

作　　者：赵连花[1] 蔡定均[2] 王蕾[1] 宋开源[2] 王静[1] 刘旭光[2] 马玉琼[1] 周奇志[2] 欧可群[1] 赵纪岚[1] 陈文玉[1] 魏焦禄[2] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎学和神经生物学教研室,四川省成都市610041 [2]成都中医药大学针灸推拿学院,四川省成都市610075

出　　处：《四川解剖学杂志》2006年第1期9-10,共2页Sichuan Journal of Anatomy

基　　金：国家自然科学基金资助(30171180)

摘　　要：目的观察针刺导引对金黄地鼠视交叉上核内c-Fos表达的影响。方法实验动物为雄性叙利亚金黄地鼠,共30只,随机分为电针组和捆绑对照组,每组15只。金黄地鼠经驯化后进入自由运行状态,在恒定黑暗第3天于近似昼夜时间(CT)9:00,15:00,21:00分别给予电针、捆绑刺激,每个时间点5只动物。各方法治疗2小时后用免疫组织化学方法检测各组金黄地鼠视交叉上核中c-Fos的免疫反应阳性细胞并对此进行计数,采用q检验方法进行统计学分析。结果30只金黄地鼠全部进入结果分析。针刺组与捆绑对照组比较在CT9:00和CT21:00可使视交叉上核内c-Fos表达显著增加(85.50±11.21,42.50±5.32,q=43.00,p<0.01;52.00±2.94,38.50±3.87,q=13.50,p<0.01)。结论针刺在CT9:00及CT21:00可使c-Fos表达增加,说明针刺对c-Fos表达的影响在主观白天及主观夜间的后半期。Objective To observe the effect of electroacupuncture on the expression of c-Fos in the suprachiasmatic nucleus（SCN） of golden hamsters. Methods A total of 30 male Syrian golden hamsters, weighing 110-140g were divided randomly into electroacupuncture and seizing control groups （n=15）. All animals moved freely following domestication . On the third day of constant darkness, animals were treated with electroacupuncture and seizing at circadian time（CT）9：00, 15： 00, and 21：00,respectively, 5 animals for each time point. At hour 2 following the treatment, the Fos-like immunoreactive neurons were observed by using immunohistochemistry and counted. The q test was used to test intergroup differences. Results All 30 hamsters were involved in the result analysis. The expression of c-Fos in SCN increased obviously in electroacupuncture group as compared with seizing control group at CT 9：00（85. 50 ±11. 21,42. 50 ±5. 32,q=43.00,p〈0.01）,as well as at CT 21：00（52. 00 ±2.94,38. 50± 3.87,q=13. 50,p〈0.01）. Conclusion The expression of c-Fos in SCN can be increased by using electroacupuncture at CT 9 ： 00 and CT 21：00. The result indicates that the electroacupuncture can affect the expression of c-Fos in SCN at subjective day and at the late half of subjective night.

关 键 词：针刺 视交叉上核 C-FOS 

分 类 号：R245[医药卫生—针灸推拿学]