正常大鼠肝脏射频消融前后外周血树突状细胞的变化及意义  

Changes of peripheral-blood dendritic cells in normal rat treated by radiofrequency ablation

作　　者：戴维德[1] 范智慧[1] 陈敏华[1] 河福金[2] 李洪民[2] 刘静贤[2] 

机构地区：[1]北京大学临床肿瘤学院北京肿瘤医院超声科,北京100036 [2]北京大学临床肿瘤学院北京肿瘤医院病理科,北京100036

出　　处：《中国介入影像与治疗学》2006年第2期137-140,共4页Chinese Journal of Interventional Imaging and Therapy

基　　金：北京市重大项目培育专项基金资助(Z0005190040431)

摘　　要：目的探讨正常大鼠肝脏射频消融前后其外周血树突状细胞(DC)数目及表型的变化和意义。方法12只健康SD大鼠均分为射频组(6只)和对照组(6只),分别在术前、术后1周抽取外周血,采用流式细胞术检测大鼠外周血树突状细胞的数量及表型分析。同时处死动物取材做病理形态学观察。结果鼠肝组织射频灶病理组织学检查显示从中心到外围呈凝固性坏死-细胞变性-肉芽组织形成的演变特点。正常大鼠的外周血单个核细胞中(0.34±0.08)%表达大鼠特异性标志OX-62,射频后1周为(0.70±0.16)%,射频后1周组与正常大鼠组比较差异有显著性。OX-6(大鼠MHCⅡ)和CD86在正常大鼠外周血树突状细胞中的阳性表达率分别为(18.20±9.40)%和(37.57±12.24)%。射频后1周分别为(19.63±5.94)%和(46.38±9.67)%。射频后1周组与正常大鼠组比较差异都没有显著性。结论射频消融促使大鼠外周血DC前体细胞数量明显增多,可能会对提高机体在免疫应答中的抗原提呈能力起到促进作用。Objective To study the change of peripheral blood dendritic cells （DC） in normal rat treated by radiofrequency ablation （RFA）. Methods Twelve healthy SD rats were separated into RFA group with 6 rats and control group with 6 rats. Peripheral blood was taken out respectively before and lw after RFA. The number and the phenotype of dendritic cells in peripheral blood were analyzed with flow-cytometry. Results Pathological examination after RFA showed the characteristic that coagulation necrosis and cellular degeneration and granulation tissue forming appeared from target center to peripheral of the target. （0.34±0.08）% of normal rat mononuclear cells in peripheral blood express OX-62, the ratio became （0.70±0.16）% after 1 week of RFA. The difference between two groups was significant. （18. 20±9.40）% and （37.57± 12.24）% of normal rat DC in peripheral blood respectively express OX-6 and CD86, the ratio became respectively （19.63±5.94）% and （46.38±9.67）% after 1 week of RFA. The difference between two groups was not significant. Conclusion RFA can make the number of peripheral blood precursor DC increasing, which may be contribute to improve the ability delivery of body to antigen to a certain extent.

关 键 词：射频消融术 大鼠 树突状细胞 

分 类 号：R73-3[医药卫生—肿瘤]