云南彝族九个短串联重复序列位点遗传多态性  

The genetic polymorphism of 9 short tandem repeat loci in Yi ethnic group of Yunnan in China

作　　者：高雅[1] 金天博[1] 赖江华[1] 郑海波[1] 李生斌[1] 

机构地区：[1]西安交通大学医学院法医学国家重点学科环境与疾病相关基因教育部重点实验室,710061

出　　处：《中华医学遗传学杂志》2006年第2期216-218,共3页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金(39970401);教育部科技基础条件平台项目(505015)~~

摘　　要：目的研究中国云南彝族人群的群体遗传结构。方法选择9个短串联重复序列（short tandem repeats，STR）位点（D3S1358、vWA、FGA、TH01、TPOX、CSFlPO、D5S818、D13S317、D7S820），采用STR复合扩增及荧光标记STR基因扫描技术，检测84名彝族无关个体血液样本。结果9个STR位点在84名云南彝族共检出69种等位片段，频率分布在0．0060-0．5060之间；检出164种基因型，频率分布在0．0119-0．4167之间。9个STR位点的基因型分布符合Hardy．Weinberg平衡定律（P〉0．05）。9个位点多态信息量分布在0．5804-0．8777之间，杂合度分布在0．6507～0．8002之间，个体识别力分布在0．7976-0．9558之间，除TPOX，TH01两个位点低于0．5外，其余7个位点的非父排除率分布在0．5207～0．8386之间。Neighbor joining（NJ）法构建彝族与云南地区其他11个少数民族的系统进化树显示：彝族首先与白族、普米族、德昂族、阿昌族、独龙族、怒族聚在一起，然后与傈僳族、傣族相聚，最后与景颇族、苗族、纳西族相聚。绪论获得了云南彝族9个STR位点的遗传多态数据，在人类群体遗传数据库建设、法医学亲权鉴定和个体识别研究及应用领域有重要价值。Objective To study the short teadem repeat（STR） genetics structure of a Chinese Yunnan Yi racial group. Methods Genetic distributions for nine STR loci were determined based on STR gene scan marked by fluorescence. Results Sixty-nine alleles and 164 kinds of genotypes were detected and identified from 84 unrelated Yi racial individuals. The corresponding gene and genotype frequencies were in 0.0060-0.5060 or 0.0119-0.4167 respectively. The expected and observed genotype frequencies of nine STR loci were in accordance with the Hardy-Weinberg equilibria. urn（ P 〉 0.05）. The statistical analyses of nine STR loci showed that PIG was distributed in 0. 5804-0. 8777, H was in 0.6507-0.8002,DP was in 0.7976-0.9558, EPP was in 0.5207-0.8386, except TPOX and THO1 loci. Conclusion Above research data enrich the Chinese genetic database, and play an important role in Chinese genetic study and in forensic application.

关 键 词：彝族 短串连重复序列 基因扫描 遗传多态性 

分 类 号：R394[医药卫生—医学遗传学] R392[医药卫生—基础医学]