果蔬中叶酸的提取分离及测定方法研究  

Study on the Extraction Purifying and Measuring Method of the Folacin in Fruits and Vegetables

作　　者：郭秀珠 黄品湖 雷海清 项秀珠 

机构地区：[1]浙江省科学院亚热带作物研究所,浙江温州325005

出　　处：《食品科学》2006年第3期183-185,共3页Food Science

摘　　要：本文研究了果蔬中叶酸的提取分离方法,对提取液、吸附剂及洗脱液设计了正交试验,结果是用热沸偏磷酸提取,活性炭吸附,3%氨-70%乙醇液洗脱,达到提取分离较完全之目的;并用荧光法测定叶酸含量,该方法回收率在95%以上,相对偏差小于2.5%,结果满意。In this paper, the extracting and purifying technology of the folacin in fruits and vegetables was studied. We designed the orthogonal test for the solvent, developing agent and sorbent. The results showed： extracted with hot boiling metaphosphoric acid, absorbed with active carbon, developed with the mixture solution of 3% ammonia and 70% anhudrous alcohol, the conclusion gained the ends of extraction and purifying comparative completeness. And we measured the content of folacin with the fluorescence spectrophotometer. The recovery ratio was beyond 95%, the relative deviation was below 2.5%. The results was satisfied.

关 键 词：叶酸 荧光法 提取分离 吸附剂 

分 类 号：O652.6[理学—分析化学]