羊草细胞变异系HR20－8的氨基酸组成水平与脯氨酸含成代谢变化的研究  

STUDIES ON AMINO ACID POOL LEVELS AND CHANGES OF PROLINGANABOLISM IN VARIANT LINE HR20-8 OF CHINESE ANEUROLEPIDIUM

作　　者：陈晖[1] 匡柏健[1] 王敬驹[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《西北植物学报》1996年第3期208-213,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金

摘　　要：离体培养，筛选获得羊草（Ａｎｅｕｒｏｌｅｐｉｄｉｕｍｃｈｉｎｅｎｓｅ（Ｔｒｉｎ．）Ｋｉｔａｇ．）抗羟脯氨酸（ＨＹＰ）细胞变异系ＨＲ２０－８，其愈伤组织中游离氨基酸和蛋白质氨基酸含量均发生了较大的变化，与供体对照比较，分别提高２．３５倍和１．４０倍，其中的脯氨酸组分在变异系体内表现异常增加，游离和蛋白质中的脯氨酸组分分别提高６．６倍和３．０倍，对脯氨酸合成途径中的γ－谷氨酸激酶的特性分析表明，变异系ＨＲ２０－８细胞内γ－谷氨酸激酶的活性比供体提高２．５倍。Obtained Chinese Aneurolepidium (Aneurolepidium chinense (Trin. )Kitag. )variantHR20-8 resistant to hydroxyproline via in vitro culture. The pools of free amino acid andprotein amino acid in HR20-8 callushave changed. Comparison of them with that indonor showed that they enhenced 2. 35 and 1. 40 times,respectively. Proline content invariant HR20-8 increased anormally. Comparison of contents of free proline and proteinproline in the variant with that in donor sheted that they enhenced 6. 6 and 3. 0 times,respectively. Examination of γ-glutamyl kinase proline biosynthesis showed that γ-gluglutamyl kinase activity in variant HR20-8 was 2. 5 times than that in donor.

关 键 词：羊草 细胞变异系 氨基酸 脯氨酸 

分 类 号：S543.901[农业科学—作物学]