Mouse Karyotype Obtained by Combining DAPI Staining with Image Analysis  

作　　者：DAI Xiaohua YANG Guangxu LIU Jingyu SONG Yunchun 

机构地区：[1]College of Life Science, Henan University, Kaifeng 475001, Henan, China [2]Department of Life Science, Western Chongqing University, Chongqing 402168, Siehuan, China [3]The Key l.aboratory of the Ministry of Education of Plant Developmental Biology, Wuhan University, Wuhan 430072, Hubei, China

出　　处：《Wuhan University Journal of Natural Sciences》2006年第2期441-446,共6页武汉大学学报（自然科学英文版）

基　　金：SupportedbyChinaPostdoctoralScienceFoundation(2003034496)andtheKeyNaturalScienceFoundationofHenanUniversity(XK03ZDSW158)

摘　　要：In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4＇, 6-diamidino-2-phenylindole （DAPI） multiple bands like （J-hands could be produced in mouse. The Meta- Morph software was then used to generate linescans of pixel intensity for the banded chromosomes from short arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established. The technique is therefore considered to he a new method for cytological studies of mouse.In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4＇, 6-diamidino-2-phenylindole （DAPI） multiple bands like （J-hands could be produced in mouse. The Meta- Morph software was then used to generate linescans of pixel intensity for the banded chromosomes from short arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established. The technique is therefore considered to he a new method for cytological studies of mouse.

关 键 词：MOUSE 4 ＇  6 ditlmidino-2-phenylindole （DA-Pl） fluorescence staining image analysis KARYOTYPE 

分 类 号：Q343.2[生物学—遗传学]