藻蓝蛋白对Au(III)原位还原与纳米Au(0)形成动态过程的谱学研究  

Spectra of in Situ Reduction of Au(III) by Phycocyanin and Dynamic Formation of Au(0)

作　　者：杨芳[1] 郭振江[1] 白燕[1] 黄峙[2] 郑文杰[1] 

机构地区：[1]暨南大学化学系 [2]暨南大学生物工程学系,广东广州510632

出　　处：《感光科学与光化学》2006年第2期118-125,共8页Photographic Science and Photochemistry

基　　金：广东省自然科学基金重点项目(05103295);广东省自然科学基金团队项目(039213)

摘　　要：在4℃、避光条件下,将藻蓝蛋白(PC)与AuCl3作用,通过UV、FS、FT-IR等谱学方法研究了PC与Au(III)作用的动态过程,结果显示:PC在615nm的特征吸收峰强度明显减弱,并随Au(III)浓度增加和时间延长单调降低;PC的荧光发射峰和荧光激发峰也均呈现衰减趋势.同时通过TEM观察到纳米Au(0)粒子形成,纳米金基本呈球形,有较窄的分布尺寸,粒径在20 nm左右,均匀地分散于PC中,这是PC原位还原Au(III)所形成的.The interaction of Au（Ⅲ） with Phycocyanin（PC） was investigated by ultraviolet-visible, fluorescence and infrared spectra under the condition of 4 ℃ and without light. The results showed that the characteristic absorption of PC at 615 nm decreased graduaUy with the increase ofAu（Ⅲ） concentration and interaction time. Accordingly, its fluorescence emission peak and two fluorescence excitation peaks also decreased. It was also observed by TEM that nano-Au（0） was formed by the PC in situ reduction. The diameter of Au（0） was about 20 nm, which was round, highly dispersed and stable in PC.

关 键 词：钝顶螺旋藻 藻蓝蛋白 光谱 纳米金 

分 类 号：Q946-33[生物学—植物学]