肝细胞癌病人肝内转移和术后复发病灶的细胞克隆来源研究  

Clonal Origin of Metastasis and Recurrence in Patients with Hepatocellular Carcinoma

作　　者：黎乐群[1] 林进令[1] 江文枢[1] 

机构地区：[1]广西医科大学第一附属医院外科,南宁530027

出　　处：《中华实验外科杂志》1996年第3期133-134,共2页Chinese Journal of Experimental Surgery

摘　　要：运用多聚酶链反应-限制性长度多态性分析(PCR-RFLP)和免疫组化技术,以抑癌基因P_(53)第7外显子24q密码子的突变和P_(53)蛋白表达,以及AFP和雌激素受体(ER)作为鉴别细胞克隆间异同的指标,对11例肝细胞癌(HCC)患者进行分析。结果发现4例经两次手术切除的原发与复发肝癌的细胞克隆来源不同,7例多个病灶的肝癌,6例多发性癌结节来源于同一细胞克隆,1例来源于不同的细胞克隆。结果表明:多个病灶的HCC大部分是单中心发生,肿瘤细胞通过肝内转移播散,而术后复发的HCC则大多数为多中心发生,由于某些致癌机制的作用,术后可反复出现肝细胞的癌变,产生新的肿瘤。Clonal origin of metastasis and recurrent lesions in 11 patients with hepatocellular carcinoma was studied by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism(PCR-RFLP) and immunohistochemical technique. The elements that we studied included codon 249 mutation in exon 7 of P53 oncogene, P53 protein expression, AFP and Estrogen Receptor(ER) in the cancer cells. The results showed that 4 cases of postoperative recurrent HCC were of different clonality, compared with their primary tumors. Of the other 7 cases of multinodular HCC, 6 cases came from the same clonality and 1 case contained two different clonality. The results indicated that most multinodular HCC generated from a single tumor cell(unicentric origin), which disseminated through metastasis within liver. But the majority of postoperative recurrent HCC were of multicentric origin. Because of the action of some carcinogens new tumors may develop postoperatively even the primary tumors have been reseeted radically.

关 键 词：肝肿瘤 肝细胞癌 转移 复发 P53点突变 细胞克隆 

分 类 号：R735.7[医药卫生—肿瘤]